Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1998-05-28
2000-03-14
Arthur, Lisa B.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
536 243, C12Q 168, C07H 2104
Patent
active
060371293
ABSTRACT:
A method of detecting the metastasis of primary breast cancer to a lymph node is provided, comprising detecting, in lymph node tissue, the presence of a nucleic acid of c-myc, PIP or keratin-19. The presence of any one of these nucleic acids in lymph node tissue is associated with metastatic breast cancer. The presence of one or more of these markers in lymph node tissue or other tissue indicates that cells from the primary tumor have migrated from the breast tissue to the lymph node or other tissue. Also provided is a method of predicting the histopathologic stage of a cancer in a patient without having to perform a histopathologic analysis, comprising detecting, in lymph node tissue from the patient, the presence of a nucleic acid of c-myc, the presence of a nucleic acid of c-myc being correlated with stage I cancer as determined by histopathology. Alternatively, the absence of a nucleic acid of PIP and the absence of a nucleic acid of keratin-19 are correlated with stage I cancer as determined by histopathology. In another embodiment, the presence of a nucleic acid of PIP is correlated with stages later than stage I cancer as determined by histopathology. Further, the presence of a nucleic acid of keratin-19 is correlated with stages later than stage I cancer as determined by histopathology. A method of predicting survival time of cancer patients is also provided, comprising detecting, in lymph node tissue from the patient, the presence of a nucleic acid of c-myc, PIP or keratin-19. The presence of a nucleic acid of any of c-myc, PIP or keratin-19 is correlated with a shorter average survival time compared with the presence of none of the nucleic acids.
REFERENCES:
Lockett et al. "Detection of Occult Breast Cancer Micrometastases in Axillary Lymph Nodes Using a Multimarker Reverse Transcriptase-Polymerase Chain Reaction Panel" J. Am. Coll. Surg. 187:9-16, 1998.
Lockett et al. "Efficacy of Reverse Transcriptase-Polymerase Chain Reaction Screening for Micrometastic Disease in Axillary Lymph Nodes of Breast Cancer Patients" Amer. Surg. 64:539-544, Jun. 1998.
Lockett et al. "Occult Breast Cancer Micrometastases: Detection in Axillary Lymph Nodes using a Multimarker Reverse . . . " Reprinterd from Surgical Forum, vol. XLVIII, pp. 861-863, 1997.
Noguchi S, Aihara R, Motamura K, et al. "Histologic Characteristics of Breast Cancers with Occult Lymph Node Metastases Detected by Keratin 19 mRNA Reverse Transcriptase-Polymerase Chain Reaction" Cancer 78:1235-1240, 1996.
Hahnel and Hahnel "Expression of the PIP/GCDFP-15 Gene and Survival in Breast Cancer" Virchows Archives--An International Journal of Pathology, 429(6):365-369, Dec. 1996.
Autiero et al. "A 17 kDa CD4-Binding Glycoprotein Present in Human Seminal Plasma and In Breast-Tumor Cells" Europ. J. Immunol. 25(5):1461-1464, May 1995.
Pagani et al. "PIP/GCDFP-15 Gene-Expression and Apocrine Differentiation in Carcinomas of the Breast" Virchows Archives--An International Journal of Pathology, 425(5):459-465, Dec. 1994.
Hahnel et al. "Hormone-Regulated Genes (pS2, PIP, FAS) in Breast-Cancer and Nontumoral Mammary Tissue" Pathobiol. 62(2);82-89, Mar.-Apr. 1994.
Bonneterre et al. "Biological and Clinical Aspects of Prolactin Receptors (PRL-R) in Human Breast Cancer" J. Steroid Biochem. Mol. Biol. 37(6):977-981, 1990.
Murphy et al. "Expression of the Gene Encoding a Prolactin-Incucible Protein by Human Breast Cancers In Vivo Correlation with Steroid Receptor Status" Cancer Res. 47(5):4160-4164, Aug. 1987.
Shiu R and Iwasiow B. "Prolactin-Inducible proteins in human breast cancer cells" J Biol Chem 260(20):11307-11313, Sep. 15, 1985.
Colby W, Chen E, Smith D, and Levinson A. "Identification and Nucleotide Sequence of a Human Locus Homologus to the v-myc Oncogene of Avian Myelocytomatosis Virus MC29" Nature 301:722-725, 1983.
Yun et al. "Keratin 19 mRNA is detectable by RT-PCr in lymph nodes of patients without breast cancer", British Journal of Cancer, vol. 76, pp. 1112-1113, 1997.
Baron Paul L.
Cole David J.
O'Brien Paul H.
Arthur Lisa B.
Medical University of South Carolina
Souaya Jehanne
LandOfFree
Multi-marker RT-PCR panel for detecting metastatic breast cancer does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Multi-marker RT-PCR panel for detecting metastatic breast cancer, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Multi-marker RT-PCR panel for detecting metastatic breast cancer will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-167898