Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1987-02-18
1990-12-04
Hill, Jr., Robert J.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
422 56, 436514, 436170, 436810, 435805, G01N 2178, G01N 3353
Patent
active
049753665
ABSTRACT:
A multi-layered element for the quantative analysis of an immuno-reactant having a water-impermeable and light-transmissible support layer; a coloring reagent layer carried by said support layer and containing a hydrophilic polymer as a binder; and a reaction layer covering said reagent layer and made of a porous matrix. When the analyte immunoreactant is an antigen, said reaction layer contains the specific antibody for the antigen, the antibody being immobilized by a first water-insoluble carrier. When the analyte immuno-reactant is an antibody, said reaction layer contains the specific antigen for the antibody. The reaction layer contains an enzyme substrate immobilized by a second water-insoluble carrier different from said first water-insoluble carrier. The coloring reagent layer contains a detection reagent composition for coupling with an enzymatic reaction product produced by the reaction beween the enzyme-labelled complex of the immuno-reactant and the enzyme substrate immobilized by the second water-insoluble carrier. Upon coupling of the detection reagent composition with the enzymatic reaction product, a color is developed, the optical density of which is measured to determine the quantity of the analyte immuno-reactant contained in a liquid sample.
REFERENCES:
patent: 3992158 (1976-11-01), Przybylowicz et al.
patent: 4258001 (1981-03-01), Pierce et al.
patent: 4806312 (1989-02-01), Greenquist
Patent Abstracts of Japan, vol. 9, No. 210 P-383 [1933] Aug. 1985, JP-A-60-71954.
Masuda Nobuhito
Miura Kenji
Sudo Yukio
Fuji Photo Film Co. , Ltd.
Hill Jr. Robert J.
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