Multi-layer immobilized enzyme compositions

Details Multi-layer immobilized enzyme compositions Multi-layer immobilized enzyme compositions

1982-10-19

1985-03-19

Naff, David M.

Chemistry: molecular biology and microbiology

Carrier-bound or immobilized enzyme or microbial cell;...

Multi-enzyme system

435176, 435207, 435234, C12N 1118, C12N 1114, C12N 938, C12N 992

Patent

active

045060150

ABSTRACT:
A high activity immobilized enzyme composite is prepared by covalently bonding a second enzyme layer to a first enzyme layer that is immobilized to a carrier. The carrier is preferably silica gel which has been activated by treatment with a strong base followed by treatment with a strong acid. The first enzyme layer is covalently bonded to the activated silica gel with an aminosilane and a polyfunctional reactant, and the second enzyme layer is covalently bonded to the first layer with a polyfunctional reactant. Third, fourth and more successive enzyme layers may be covalently bonded. The composite has high activity per unit volume, superior stability and good half-life.

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Messing, R. A., Immobilized Enzymes For Industrial Reactors, Academic Press, N.Y., 1975 (pp. 91-95).
Olson et al., J. Ag. Food Chem. 21, 440 (1973).

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