Radiant energy – Luminophor irradiation
Patent
1997-08-29
1999-03-09
Westin, Edward P.
Radiant energy
Luminophor irradiation
2504591, 356317, G01J 158, G01N 2125, G01N 3348
Patent
active
058804749
ABSTRACT:
A multi-laser flow-cytometry system provides for inter-location emissions crosstalk cancellation, through either pulse subtraction or gating, to avoid false triggering based on shadow (pre- and post-) pulses that occur when red-excited red emissions are detected by a photodetector arranged to detect blue-excited red emissions. This overcomes a problem of undercounting of counting beads that led to misdeterminations of cell counts when blue-excited red fluorescence detections were used as a trigger. The approach also provides for more accurate quantitative data regarding fluorochrome emissions amplitudes.
REFERENCES:
patent: 4867908 (1989-09-01), Recktenwald et al.
patent: 5682038 (1997-10-01), Hoffman
James C. S. Wood et al.: "Dual Laser Excitation Flow Cytometry: The State-of-the-Art". Book entitled Flow Cytometry: Advanced research and Clinical Applications, vol. 1, 1989 Boca Raton, Florida, pp. 6-7, 18-25, Illumination and Optics Components, pp. 28-33, Analogue Signal Processing, Data Acquisition, and Display.
Ger Van Den Engh et al.: "Parallel Processing Data Acquisition System for Multilaser Flow Cytometry and Cell Sorting." Cytometry vol. 10, pp. 282-293 (1989), 1989 Alan R. Liss, Inc., see the whole document.
George B. J. Dubelaar.: "Optical Plankton analyser: A Flow Cytometer for Plankton Analysis, II: Specifications 1" Cytometry vol. 10, pp. 529-539 (1989), 1989 Alan R. Liss, Inc., see the whole document.
R. D. Hiebert et al.: "Electronic for Flow Cytometers and Sorters" Flow Cytometery: Instrumentationa DN Data Analysis vol. 4, pp. 129-162, Copyright 1985 by Academic Pres Inc. (London) Ltd., ISBN 0-12-712150-1, see the whole document.
Howard M. Shapiro et al.: "Cytomat-R: A Computer-Controlled Multiple Laser Source Multiparameter Flow Cytophotometer System" The Journal of Histochemistry and Cytochemistry, vol. 25, No. 7, pp. 836-844, 1977 Copyright 1977 by The Histochemical Society, Inc., Printed in the U.S.A., see the whole document.
Hoffman Robert A.
Norton Pierce Owen
Becton Dickinson and Company
Capello Susan A.
Hanig Richard
Lee Eric M.
Wark Allen W.
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