Multi-domain DNA ligands for protein and nucleic acid affinity c

Details Multi-domain DNA ligands for protein and nucleic acid affinity c Multi-domain DNA ligands for protein and nucleic acid affinity c

1995-06-07

1996-09-24

Jones, W. Gary

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 911, 435 912, 435 914, 536 221, 536 242, C12Q 168, C12P 1934, C07H 2102

Patent

active

055590012

ABSTRACT:
The present invention is directed to a multi-domain oligonucleotide ligand having multiple functions conferred by each of the domains. The ligand is bound to a solid phase matrix for use in both mRNA-affinity chromatography and as a priming matrix for generating matrix-bound cDNA from the mRNA bound to the matrix. The resultant cDNA was tested and found to function as a solid-phase template for PCR. This solid-phase template approach to PCR is demonstrated with oligo-d(T) paper. The functional domains are defined by polynucleotide sequences on the ligand to confer the different functions.

REFERENCES:
patent: 4683202 (1987-07-01), Mullis
patent: 4797355 (1989-01-01), Stabinsky
patent: 5200314 (1993-04-01), Urdea
patent: 5437976 (1995-08-01), Utermohlen
Stamm and Brosius (1991) Sanchored PCR: PCR with cDNA coupled to a solid se, Nucleic Acids Research, 19:1350.
Venetianer and Leder (1974) Enzymatic Synthesis of Solid Phase-Bound DNA Sequences Corresponding to Specific Mammalian Genes, Proc. Natl. Acad. Sci. USA 71:3892-3895.
Seed (1982) Nucleic Acids Research, 10:1799-1810.
Noyes et al. (1975) Nucleic Acid Hybridization Using DNA Covalently Coupled to Cellulose, Cell 5:301-310.
Gilham (1971) Methods in Enzymology 21:191-197.

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