Multi-domain DNA ligands bound to a solid matrix for protein and

Details Multi-domain DNA ligands bound to a solid matrix for protein and Multi-domain DNA ligands bound to a solid matrix for protein and

1991-08-08

1995-08-01

Walsh, Stephen G.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

536 231, 536 2433, 536 254, 935 19, 935 20, 935 21, C12Q 168, C07H 2102, C07H 2104, C12N 1511

Patent

active

054379768

ABSTRACT:
The present invention is directed to a multi-domain oligonucleotide ligand having multiple functions conferred by each of the domains. The ligand is bound to a solid phase matrix for use in both mRNA-affinity chromatography and as a priming matrix for generating matrix-bound cDNA from the mRNA bound to the matrix. The resultant cDNA was tested and found to function as a solid-phase template for PCR. This solid-phase template approach to PCR is demonstrated with oligo-d(T) paper. The functional domains are defined by polynucleotide sequences on the ligand to confer the different functions.

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