Multi-dimensional proteomic analysis method

Chemistry: electrical and wave energy – Processes and products – Electrophoresis or electro-osmosis processes and electrolyte...

Reexamination Certificate

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C204S606000, C204S461000

Reexamination Certificate

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07347922

ABSTRACT:
A multi-dimensional proteomic analysis method utilizing cationic electrophoresis is described. The method includes separating proteins in one direction using cationic electrophoresis and separating the proteins in a second orthogonal direction using other electrophoresis separation methods such as denaturing electrophoresis and electrophoresis subsequent to proteolytic cleavage or isofocussing. The two dimensional array may be used to determine various protein-protein interactions in a sample.

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H. Sun et al. “Using native gel in two-dimensional PAGE for the detection of protein interactions in protein extract” J. Biochem. Biophys. Methods. 39, 143-151. (1999).

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