Multi-color labeling of different antigens in a biological syste

Details Multi-color labeling of different antigens in a biological syste Multi-color labeling of different antigens in a biological syste

1985-09-27

1990-04-17

Warden, Robert J.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving antigen-antibody binding, specific binding protein...

435 1, 435 4, 435 29, 435810, 424 3, 436503, 436536, 436546, 436547, 436548, 436800, 436805, 436808, G01N 3353

Patent

active

049180002

ABSTRACT:
A method of multi-color labeling at least two antigens present in a tissue such as non-liquid tissue or body fluids, with the aid of different antibodies is improved by introducing between the labeling of the first antigen and the labeling of the second antigen, and between labeling of the second and that of any further antigen, at least once a treatment with a non-immune normal serum and optionally other agents blocking any binding sites remaining free in the tissue after a last-preceding labeling step.

REFERENCES:
patent: 4672028 (1987-06-01), Olson

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