Radiant energy – Luminophor irradiation
Patent
1986-08-21
1988-05-17
Howell, Janice A.
Radiant energy
Luminophor irradiation
2504591, 2504611, 2504612, 356318, G01N 2164
Patent
active
047452854
ABSTRACT:
A method for determining one or more characteristics of particles using multiple fluorescence analysis comprises directing an incident light beam at the particles under analysis. The particles include at least three fluorescent markers each having different emission spectra. The incident light beam causes the excitation of the markers by light at a single wavelength whereby different wavelengths of fluorescence are emitted from the particles. Different fluorescence emissions associated with the particles under analysis are simultaneously detected. This method further includes associating the detected fluorescence with one or more characteristics of the particles. An apparatus is also part of the present invention for carrying out the aforementioned method.
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patent: 3826364 (1974-07-01), Bonner et al.
patent: 4520110 (1985-05-01), Stryer et al.
patent: 4542104 (1985-09-01), Stryer et al.
patent: 4599307 (1986-07-01), Saunders et al.
M. R. Loken, D. R. Parks, and L. A. Herzenberg, "Two-Color Immunofluorescence Using a Fluorescence-Activated Cell Sorter" The Journal of Histochemistry and Cytochemistry, vol. 25, No. 7 (1977), pp. 899-907.
Chen Chia H.
Recktenwald Diether J.
Becton Dickinson and Company
Hannaher Constantine
Howell Janice A.
Rodrick Richard J.
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