Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving virus or bacteriophage
Patent
1988-06-10
1992-12-22
Nucker, Christine M.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving virus or bacteriophage
435 71, 435792, 435793, 435794, 435795, 435971, 435974, 435975, 43524027, 5303883, 436548, C12Q 170, G01N 3353, C12N 520, C07K 1528
Patent
active
051733994
ABSTRACT:
The present invention provides monoclonal antibodies demonstrating specific reactivity with HIV-1 p24. One monoclonal antibody designated 31-42-19 recognizes an unique epitope on HIV-1 p24 that is not immunogenic in humans. 31-42-19 also reacts with an antigenically cross reactive epitope on HIV-2 p24. Another monoclonal antibody designated 31-90-25 recognizes an epitope within a highly immunogenic region of HIV-1 p24. The present invention also provides cell lines capable of producing these monoclonal antibodies. The invention also includes a highly sensitive enzyme immunoassay for the detection of HIV-1 p24 in biological fluids, using a monoclonal antibody mixture. The present invention further provides methods for the use of these monoclonal antibodies for the detection of anti-HIV-1 p24 antibodies and HIV-2 p24 antigen in biological samples.
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Devare Sushil G.
Hunt Jeffrey C.
Mehta Smriti U.
Abbott Laboratories
Eggert Joan D.
Nucker Christine M.
Porembski Priscilla E.
Woodward M. P.
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