Multicellular living organisms and unmodified parts thereof and – Nonhuman animal – The nonhuman animal is a model for human disease
Reexamination Certificate
2011-06-14
2011-06-14
Singh, Anoop (Department: 1632)
Multicellular living organisms and unmodified parts thereof and
Nonhuman animal
The nonhuman animal is a model for human disease
C800S018000, C800S021000, C800S022000, C800S003000
Reexamination Certificate
active
07960606
ABSTRACT:
An animal model has been developed where the animals can survive myocardial infarctions caused by diet-induced coronary atherosclerosis, and live with chronic heart failure. This animal model is a result of reduced activity of scavenger receptor class BI (SR-BI) and ApoE and the inducible activity of the Mx1-Cre gene. In a preferred embodiment, the model is a result of crossbreeding two transgenic mouse lines: a knockout of SR-BI (SRBI−/−) and an impaired ApoE expressor (Apoeh/h) to generate a strain referred to as Apoeh/hSRB1−/−mice, which is then crossbred to mice that carry the inducible Mx1-Cre transgene. The Apoeh/hSRB1−/−mouse model is genetically modified, enabling the offspring to rapidly and permanently lower their high blood cholesterol levels caused by dietary challenge. The ability to rapidly and permanently lower blood cholesterol levels in these mice stops and may cause the regression of occlusive coronary atherosclerosis restoring blood flow to the heart, allowing the mice to survive from myocardial infarction and live with chronic heart failure.
REFERENCES:
patent: 3625214 (1971-12-01), Higuchi et al.
patent: 4789734 (1988-12-01), Pierschbacher
patent: 4906474 (1990-03-01), Langer et al.
patent: 4925673 (1990-05-01), Steiner et al.
patent: 6437215 (2002-08-01), Krieger et al.
patent: 2002/0108131 (2002-08-01), Krieger et al.
patent: 2002/0194628 (2002-12-01), Weisgraber et al.
patent: 2005/0223420 (2005-10-01), Krieger et al.
patent: WO 02/074967 (2002-09-01), None
patent: WO 2005/011494 (2005-02-01), None
Clark et al Nature Reviews: Genetics. 2003,. 825-833.
Niemann et al Rev. Sci, Tech. Off. Int. Spiz. 2005, (24), 285-298.
Wheeler Theriogeneology. 2001, (56), 1345-1369.
Denning et al Cloning and Stem Cells, 2001, 3(4), 221-231.
Yanagimach et al Molecular and Cellular Endocrinology, 2002, 187, 241-248.
Oback and Wells Cloning and Stem Cells, 2002, 4, 169-174.
Wolf et al Journal of Biotechnology 1998, 65: 99-110.
Stice et al Therigeneology, 1998,49: 129-138.
Wooddell et al, Biochem Biophys Res Commun. Aug. 19, 2005;334(1):117-27.
Carmell MA Nat Struct Biol. 2003; 10(2): 91-92.
Sigmund, C Arterioscler. Thromb. Vasc. Biol.2000, 1425-1429.
Griffiths et al Microscopy Research and Technique 1998, 41: 344-358.
Tailleux et al Trends Pharmacol Sci. 2003:24(10):530-4.
Schoonjans et al Stem Cells, 2003; 21:90-97.
Wolfer et al Trends in Neuroscience, 2002, 25 (7): 336-340.
Gerlai, Trends Neurosci. 1996, 19(5):177-81.
Holschneider et al. Int J Devl Neuroscience, 2000, 18: 615-618.
Braun, et al., “Probucol prevents early coronary heart disease and death in the high-density lipoprotein receptor SR-BI/apolipoprotein E double knockout mouse”,Proc Natl Acad Sci., 100:7283-7288 (2003).
Braun, et al., “Loss of SR-BI expression leads to the early onset of occlusive atherosclerotic coronary artery disease, spontaneous myocardial infarctions, severe cardiac dysfunction, and premature death in apolipoprotein E-deficient mice”,Circ. Res., 90(3):270-6 (2002).
Caligiuri, et al., “Myocardial infarction mediated by endothelin receptor signaling in hypercholesterolemic mice”,Proc. Natl. Acad. Sci. U.S.A., 96(12):6920-4 (1999).
Clark and Whitelaw, “A future for transgenic livestock”,Nat Rev Genet. Oct. 4(10):825-33) (2003).
Cooper, “Hepatic uptake of chylomicron remnants”,J Lipid Res, 38(11):2173-2192 (1997).
Dansky, et al., “T and B lymphocytes play a minor role in atherosclerotic plaque formation in the apolipoprotein E-deficient mouse”,Proc. Natl. Acad. Sci. U.S.A., 94(9):4642-6 (1997).
Dorsett and Tuschl, “siRNAs: applications in functional genomics and potential as therapeutics.”Nat Rev Drug Discov., 3(4):318-29 (2004).
Gao, et al., “Serial echocardiographic assessment of left ventricular dimensions and function after myocardial infarction in mice”,Cardiovasc. Res., 45(2):330-8 (2000).
Gardin, et al., “Echocardiographic assessment of left ventricular mass and systolic function in mice”,Circ. Res., 76(5):907-14 (1995).
Guo, et al., “Temporal Control of Cre Recombinase-mediated in Vitro DNA Recombination by Tet-on Gene Expression System”,Acta Biochimica et Biophysica Sinica, 37(2):133-138 (2005).
Kemp, et al., “Elimination of background recombination: somatic induction of Cre by combined transcriptional regulation and hormone binding affinity.”, Nucleic Acids Res. Jul. 1, 2004;32(11):e92. (2004).
Kocher, et al., “Targeted disruption of the PDZK1 gene in mice causes tissue-specific depletion of the high density lipoprotein receptor scavenger receptor class B type I and altered lipoprotein metabolism”,J Biol Chem278(52):52820-52825 (2003).
Kühn, “Inducible gene targeting in mice”,Science269 (5229):1427-1429 (1995).
Liao, et al., “Echocardiographic assessment of LV hypertrophy and function in aortic-banded mice: necropsy validation”,Am. J. Physiol. Heart Circ. Physiol., 282(5):H1703-8 (2002).
Mahley and Ji , “Remnant lipoprotein metabolism: key pathways involving cell-surface heparan sulfate proteoglycans and apolipoprotein E ”,J Lipid Res, 40:1-16 (1999).
Miettinen, et al., “Abnormal lipoprotein metabolism and reversible female infertility in HDL receptor (SR-BI)-deficient mice”,J Clin Invest., 108(11):1717-22 (2001).
Nieland, et al., “Discovery of chemical inhibitors of the selective transfer of lipids mediated by the HDL receptor SR-BI”,Proc Natl Acad Sci U S A. 99(24):15422-7 (2002).
Niemann, et al., “Transgenic farm animals: Present and future”,Rev. Sci. Tech. Off. Int. Spiz., 24:285-298 (2005).
Nishimura, et al., “Autoimmune dilated cardiomyopathy in PD-1 receptor-deficient mice”,Science, 291(5502):319-2 (2001).
Prelle, et al., “Pluripotent stem cells—model of embryonic development, tool for gene targeting, and basis of cell therapy”,Anat. Histol. Embryol., 31(3):169-86 (2002).
Raffai and Weisgraber, “Hypomorphic apolipoprotein E mice: a new model of conditional gene repair to examine apolipoprotein E-mediated metabolism”,J. Biol Chem. 277(13)11064-11068 (2002).
Raffai, et al., “Introduction of human apolipoprotein E4 “domain interaction” into mouse apolipoprotein E”,Proc Natl Acad SciUSA, 98(20):11587-11591 (2001).
Reardon, et al., “Effect of immune deficiency on lipoproteins and atherosclerosis in male apolipoprotein E-deficient mice”,Arterioscler. Thromb. Vasc. Biol., 21(6):1011-6 (2001).
Rigotti, et al ., “The role of the high-density lipoprotein receptor SR-BI in the lipid metabolism of endocrine and other tissues”,Endocr Rev., 24(3):357-87 (2003).
Rigotti, et al., “A targeted mutation in the murine gene encoding the high density lipoprotein (HDL) receptor scavenger receptor class B type I reveals its key role in HDL metabolism”,Proc Natl Acad Sci USA, 94:12610-12615 (1997).
Rohlmann, et al., “Inducible inactivation of hepatic LRP gene by cre-mediated recombination confirms role of LRP in clearance of chylomicron remnants”,J. Clin. Invest., 101: 689-695 (1998).
Roselaar, et al., “Lymphocyte populations in atherosclerotic lesions of apoE −/− and LDL receptor −/− mice. Decreasing density with disease progression”,Arterioscler. Thromb. Vasc. Biol., 16(8):1013-8 (1996).
Roth, et al., “Impact of anesthesia on cardiac function during echocardiography in mice”,Am. J. Physiol. Heart Circ. Physiol,.282(6):H2134-40 (2002).
Schneider, et al., “Differential, inducible gene targeting in renal epithelia, vascular endothelium, and viscera of Mx1Cre mice”,Am. J. Physiol. Renal Physiol., 284(2):F411-F417 (2002).
Tanaka, et al., “Transthoracic echocardiography in models of cardiac disease in the mouse”,Circulation, 94(5):1109-17 (1996).
Takuma, et al., “Anesthetic inhibition in ische
Raffai Robert L.
Weisgraber Karl
Pabst Patent Group LLP
Singh Anoop
The J. David Gladstone Institutes
The Regents of the University of California
The United States of America as represented by the Department of
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