Motionless mixer as cell culture propagator

Details Motionless mixer as cell culture propagator Motionless mixer as cell culture propagator

1981-06-22

1983-11-15

Penland, R. B.

Chemistry: molecular biology and microbiology

Apparatus

Mutation or genetic engineering apparatus

435284, 435313, C12M 300

Patent

active

044156707

ABSTRACT:
Use of a static mixing system element as a tissue culture propagator. The element comprises an assembly of parallel sheets shaped to provide a plurality of mixing cells wherein fluid entering a cell from two separate inlet channels is rearranged due to shearing and extensional forces and divided into two new outlet streams each of which leaves the mixing cell in a different direction from either inlet stream. The configuration of the device provides uniform flow across all surfaces for precise control of growth conditions and maximum cell growth.

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patent: 3948732 (1976-04-01), Haddad et al.
patent: 3976547 (1976-08-01), McAleer et al.
Rudiger, "Methods in Cell Biology", Academic Press, (1975), vol. 9, pp. 13-23.
McCoy et al., "Class Helix Perfusion Chamber for Massive Growth of Cells in Vitro", Proc. Soc. Biol. Med., vol. 109, (1962), pp. 235-237.

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