Motility scanner and method

Optics: measuring and testing – For light transmission or absorption – Of fluent material

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Details

350509, 350523, 356244, 382 6, 435291, G01N 2159, G01N 2145, G01N 33487

Patent

active

048969672

ABSTRACT:
An improved motility scanner is disclosed for characterizing the motion of sperm, bacteria, particles suspended in flowing fluids and the like. The motility scanner includes an improved optical system, a source of illumination for the system and radiation sensing means including electronic image reversal means coupled to the system. A disposable specimen holder allows external loading thereof and its positioning on a heated specimen support. The illumination source functions as a collimator in directing all of the emanating illumination onto the specimen. Both directly transmitted light and light scattered by the specimen are received by an imaging lens and are both focussed thereby onto pixels of a light sensitive device. A movable plate provided with a retarding member is designed to be located at the plane conjugate to the plane of the small source aperature. The directly transmitted light is transmitted through the retarding member and/or the attenuating member. The light scattered by the specimen, however, travels for the most part through the plate in areas not covered by the retarding and/or attenuating members.

REFERENCES:
patent: 4407569 (1983-10-01), Piller et al.
patent: 4690678 (1987-09-01), Douglas-Hamilton
Edmonds "Light-Emiting Diode Optical Probe For Epitaxial Film Characterization" IBM Tech. Disc. Bull. vol. 14 #9, Feb. 1972 pp. 2610-2611.
Wren "Understanding & Using The Phase Microscope" Unitron Educational Pub., pp. 32-36.
Bausch & Lomb Catalog D-104, pp. 5-7.
Unitron-Phase-Microscope MP-Series.
"Le System Sperdyn"-Mesurez L'Activite Des Spermatozoides by Gaclma, France.
Von F. Zernike "Das Phasenkontrastverfahren . . ." Physik Zeitsch XXXVI, 1935, pp. 848-851.
F. Zernike "Phase Contrast, A New Method for the Microscopic Observation of Transparent Objects," Physica IX, No. 7 (Jul. 1942) 686-698.
K. Yamamoto et al., "Some Improvements in the Phase Contrast Microscope", Journal of Microscopy, vol. 129, Pt. 1. Jan. 1983, pp. 49-62.

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