Mosaic protein and restriction endonuclease assisted ligation me

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving virus or bacteriophage

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530324, 530826, C12Q 170

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060307714

ABSTRACT:
A mosaic protein comprising a variety of immunoreactive antigenic epitopes from several genotypes of hepatitis C virus. The mosaic protein provides a sensitive and specific immunological hepatitis detection assay. A restriction enzyme assisted ligation method of making an artificial gene permits controlled construction of mosaic proteins, and allows confirmatory expression of the intermediate gene products.

REFERENCES:
patent: 5106726 (1992-04-01), Wang
patent: 5302507 (1994-04-01), Chiba et al.
patent: 5436126 (1995-07-01), Wang
patent: 5436318 (1995-07-01), Reyes et al.
patent: 5443965 (1995-08-01), Reyes et al.
patent: 5538865 (1996-07-01), Reyes et al.
patent: 5563032 (1996-10-01), Fields et al.
patent: 5574132 (1996-11-01), Lacroix
patent: 5625034 (1997-04-01), Liao et al.
"Detection of Antibodies to Hepatitis C Virus (HCV) Structural Proteins in Anti-HCV-Positive Sera by an Enzyme-Linked Immunosorbent Assay Using Synthetic Peptides as Antigens"; Chuzo Ishida et al. Journal of Clinical Microbiology, vol. 31, No. 4, Apr. 1993, pp. 936-940.
"E2 and NS5: New Antigens for Detection of Hepatitis C Virus Antibodies"; H.L. Zaaijer et al., Journal of Medical Virology, 44:395-397, Apr. 25, 1994.
"Improved Diagnosis of Chronic Hepatitis C Virus Infection by Detection of Antibody to Multiply Epitopes: Confirmation by Antibody to Syunthetic Oligopeptides", D. Brown et al., Journal of Medical Virology, 38:167-171, Apr. 3, 1992.
"Preformed Antigen-Antibody Complex to Detect Antibodies to Hepatitis C Virus", Dinesh Shah, Clinical Chemistry, vol. 41, No. 9, 1357-58, 1995.
"Synthetic gene for the hepatitis C virus nucleocapsid protein", Y.E. Khudyakov et al., Nucleic Acids Research, vol. 21, No. 11, pp. 2747-2754, Apr. 30, 1993.
"IgM and IgG Antbodies to Hepatitis E Virus (HEV) Detected by an Enzyme Immunoassay Based on an HEV-Specific Artificial Recombinant Mosaic Protein", M.O. Favorov, Journal of Medical Virology, 50:50-58, May 9, 1996.
"Artificial Mosaic Protein Containing Antigenic Epitopes of Hepatitis E Virus"; Yury E. Khudyakov et al., Journal of Virology, vol. 68, No. 11, Nov. 1994, pp. 7067-7074.
"Solid-phase enzyme-linked immunosorbent assay for hepatitis E virus IgG and IgM antibodies utilizing recombinant antigens and synthetic peptides", George J. Dawson et al., Journal of Virological Methods, 38 (1992) pp. 175-186.
"Study on Reliability of Commercially Available Hepatitis C Virus Antibody Tests", H.H. Feucht et al., Journal of Clinical Microbiology, vol. 33, No. 3, Mar. 1995, pp. 620-624.
"Influence of Viraemia and Genotype Upon Serological Reactivity in Screening Assays for Antibody to Hepatitis C Virus", S.K. Dhaliwal et al., Journal of Medical Virology, 48:184-190 (1996).
"Improved serodiagnosis of hepatitis C virus infection with synthetic peptide antigen from capsid protein", Barbara Hosein et al., Proc. Natl. Acad. Sci. USA, vol. 88, May 1991, pp. 3647-3651.
"Hepatitis E. virus (HEV): The novel agent responsible for enterically transmitted non-A, non-B hepatitis", Gregory R. Reyes et al., Gastroenterologia Japonica, vol. 26, Suppl. 3, Jul. 1991, pp. 142-147.
"T C or Not to C: These are the Questions", Harvey J. Alter, Blood, vol. 85, No. 7, Apr. 1, 1995; pp. 1681-1695.
"Genotype-Dependent Serologic Reactivities in Patients Infected with Hepatitis C Virus in the United States", Nizar N. Zein, M.D. et al., Mayo Clin. Proc., 1995; 70:449-452.
"Improved serologic detection of hepatitis C virus with a paramagnetic microparticle assay using multiple antigenic sequences", D. Leahy et al., Transufsion, vol. 32, No. 6, 1992, pp. 548-553.
"Genetic organization and diversity of the hepatitis C virus", Q.L. Choo et al., Proc. Natl. Acad. Sci. USA, vol. 88 Mar. 1991, pp. 2451-2455.
Bukh et al., Proc. Natl. Acad. Sci. USA, 91:8239-8243, Aug. 1994.
Chien et al., Proc. Natl. Acad. Sci USA, 89:10011-10015, Nov. 1992.
Fields et al., Clinical and Diagnostic Virology 5:167-179, Jul. 1996.
Ruedinger, et al., Abstracts of the 97th General Meeting of the American Society for Microbiology, Abstracts in Clinical And Diagnostic Immunology, #V-54, p. 583, May 1997.
Zhang et al., Journal of Medical Virology, 45:50-55, 1995.
Khudyakov, Yury et al., "New Frontiers in Protein Engineering: Artificial Mosaic Antigens", 17.sup.th International Congress of Biochemistry and Molecular Biology in Conjunction with the Annual Meeting of the American Society for Biochemistry and Molecular Biology, San Francisco, California, USA, Aug. 24-29, 1997, FASEB Journal 11 (9), 1997, abstract No. 1574.
Chokri, Bahoul et al, "DNA-Based Immunization for Exploring the Enlargement of Immunological Cross-Reactivity Against the Lyssaviruses", Vaccine, vol. 16, No. 4, Feb. 1998, pp. 417-425.

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