Mortierella alpina C 16/18 fatty acid elongase

Details Mortierella alpina C 16/18 fatty acid elongase Mortierella alpina C 16/18 fatty acid elongase

2005-10-19

2008-12-30

Prouty, Rebecca (Department: 1652)

Chemistry: molecular biology and microbiology

Micro-organism, per se ; compositions thereof; proces of...

Bacteria or actinomycetales; media therefor

C435S193000, C435S069100, C435S254200, C435S254100, C435S252100, C435S255200, C435S254220, C435S257100, C536S023200

Reexamination Certificate

active

07470532

ABSTRACT:
The present invention relates to a fungal C16/18fatty acid elongase that is able to catalyze the conversion of palmitate (16:0) to stearic acid (18:0). Specifically, the nucleotide sequence of aMortierella alpinaC16/18fatty acid elongase is provided (designated as “ELO3”). Methods of increasing microbial oil production, increasing carbon flux into the polyunsaturated fatty acid biosynthetic pathway and increasing the content of polyunsaturated fatty acids by over-expression of the C16/18fatty acid elongase are described herein. Most desirably, the substrate specificity of the instant ELO3 will be particularly useful to enable accumulation of long-chain polyunsaturated fatty acids in oleaginous yeast, such asYarrowia lipolytica.

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