More virulent biotype isolated from wild-type virus

Details More virulent biotype isolated from wild-type virus More virulent biotype isolated from wild-type virus

1989-06-30

1992-07-21

Kepplinger, Esther L.

Chemistry: molecular biology and microbiology

Virus or bacteriophage, except for viral vector or...

435 5, 424 93T, C12N 700

Patent

active

051322200

ABSTRACT:
A method has been found to select the most virulent biotypes from a wild-type entomopathogenic virus by selection of desirable characteristics after serial passages through in vivo and in vitro systems. Using this method, a strain of Lymantria dispar NPV virus was isolated which had increased biological activity and increased speed of kill.

REFERENCES:
patent: 4337314 (1982-06-01), Oeschger et al.
patent: 4514497 (1985-04-01), Kit et al.
patent: 4535060 (1985-08-01), Comai
patent: 4870023 (1989-09-01), Fraser et al.
Martin Shapiro et al., "Comparative Infectivities of Gypsy Moth (Lepidoptera: Lumantriidae) Nucleopolyhedrosis Virus Isolates from North America, Europe, and Asia," J. Econ. Entomol. 77: 153-156 (1984).
Martin Shapiro and Edward Dougherty, "Selection of Active Strains of the Gypsy Moth Nuclearpolyhedrosis Virus," Proc. Symp.: Microbial Control of Spruce Budworm and Gypsy Moths, GTR-NE-100, pp. 115-122 (1985).
Martin Shapiro, "In vivo Selection of More Virulent Biotypes of the Gypsy Moth NPV," Soc. Invertebr. Pathol. XX Annu. Mtg. Abstr. 92, p. 85, Jul. 20-24 (1987).

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