Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Using tissue cell culture to make a protein or polypeptide
Reexamination Certificate
2006-11-28
2006-11-28
Maier, Leigh C. (Department: 1623)
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Using tissue cell culture to make a protein or polypeptide
C435S071100, C536S001110, C536S124000
Reexamination Certificate
active
07141394
ABSTRACT:
A novel C-glycoside of isopropylthiogalactoside (IPTG), isobutyl-C-galactoside (IBCG), is described. IBCG may be used as an IPTG substitute for increased induction of protein expression of plasmid-based genes for the production of recombinant proteins under the control of the lac promoter. IBCG offers the advantage over IPTG of being stable at ambient temperature.
REFERENCES:
patent: 6174867 (2001-01-01), Hindsgaul
Baneyx, F. “Recombinant protein expression . . . ” Curr. Opin. Biotechnol. (1999) vol. 10, pp. 411-421.
Marquez, F. et al “Aplicacion de la reaccion de grignard . . . ” Anales de Quimica (1983) vol. 79, pp 428-431.
Wikipedia entry for “Lac Operon” http://en.wikipedia.org/wiki/Lac—operon—retrieved Dec. 22, 2005.
USBiological product entry for “IPTG Dioxane Free” http://www.usbio.net/Product.aspx?Prodld=108—retrieved Dec. 22, 2005.
Leveau, Johan H. J., et al.; “Predictive and Interpretive Simulation of Green Fluorescent Protein Expression In Reporter Bacteria” Journal of Bacteriology, Dec. 2001, p. 6752-6762.
Ko Kwang-Seuk
Pohl Nicola Lucia
Iowa State University & Research Foundation, Inc.
Maier Leigh C.
McKee Voorhees & Sease, P.L.C.
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