Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving virus or bacteriophage
Patent
1995-06-05
1998-10-13
Kemmerer, Elizabeth C.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving virus or bacteriophage
435 681, 435 691, 435 697, 435 698, 4351721, 4352351, 435239, 436802, 530350, 5303871, 5303879, 536 241, C12Q 170, C12N 1563, C12N 702, C07K 1600
Patent
active
058210474
ABSTRACT:
A method for selecting novel proteins such as growth hormone and antibody fragment variants having altered binding properties for their respective receptor molecules is provided. The method comprises fusing a gene encoding a protein of interest to the carboxy terminal domain of the gene III coat protein of the filamentous phage M13. The gene fusion is mutated to form a library of structurally related fusion proteins that are expressed in low quantity on the surface of a phagemid particle. Biological selection and screening are employed to identify novel ligands useful as drug candidates. Disclosed are preferred phagemid expression vectors and selected human growth hormone variants.
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Bass Steven
Garrard Lisa J.
Greene Ronald
Henner Dennis J.
Lowman Henry B.
Genentech Inc.
Kemmerer Elizabeth C.
Lathrop Brian
Schwartz Timothy R.
Winter Daryl B.
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