Monoterpene synthases from common sage (Salvia officinalis)

Details Monoterpene synthases from common sage (Salvia officinalis) Monoterpene synthases from common sage (Salvia officinalis)

1997-09-25

1999-04-06

Wax, Robert A.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Transferase other than ribonuclease

536 232, 536 236, 530350, 435 691, 4352523, 4353201, C12N 910, C12N 1500, C12N 120, C07H 2104

Patent

active

058916970

ABSTRACT:
cDNAs encoding (+)-bornyl diphosphate synthase, 1,8-cineole synthase and (+)-sabinene synthase from common sage (Salvia officinalis) have been isolated and sequenced, and the corresponding amino acid sequences has been determined. Accordingly, isolated DNA sequences (SEQ ID No:1; SEQ ID No:3 and SEQ ID No:5) are provided which code for the expression of (+)-bornyl diphosphate synthase (SEQ ID No:2), 1,8-cineole synthase (SEQ ID No:4) and (+)-sabinene synthase SEQ ID No:6), respectively, from sage (Salvia officinalis). In other aspects, replicable recombinant cloning vehicles are provided which code for (+)-bornyl diphosphate synthase, 1,8-cineole synthase or (+)-sabinene synthase, or for a base sequence sufficiently complementary to at least a portion of (+)-bornyl diphosphate synthase, 1,8-cineole synthase or (+)-sabinene synthase DNA or RNA to enable hybridization therewith. In yet other aspects, modified host cells are provided that have been transformed, transfected, infected and/or injected with a recombinant cloning vehicle and/or DNA sequence encoding (+)-bornyl diphosphate synthase, 1,8-cineole synthase or (+)-sabinene synthase. Thus, systems and methods are provided for the recombinant expression of the aforementioned recombinant monoterpene synthases that may be used to facilitate their production, isolation and purification in significant amounts. Recombinant (+)-bornyl diphosphate synthase, 1,8-cineole synthase and (+)-sabinene synthase may be used to obtain expression or enhanced expression of (+)-bornyl diphosphate synthase, 1,8-cineole synthase and (+)-sabinene synthase in plants in order to enhance the production of monoterpenoids, or may be otherwise employed for the regulation or expression of (+)-bornyl diphosphate synthase, 1,8-cineole synthase and (+)-sabinene synthase, or the production of their products.

REFERENCES:
Croteau, R., and Karp, F., "Biosynthesis of Monoterpenes: Preliminary Characterization of Bornyl Pyrophosphate Synthetase from Sage (Salvia Officinalis) and Demonstration that Geranyl Pyrophosphate Is the Preferred Substrate for Cyclization," Arch. Biochem. Biophys. 198(2):512-522 (1979).
Croteau, R., and Karp, F., "Biosynthesis of Monoterpenes: Hydrolysis of Bornyl Pyrophosphate, an Essential Step in Camphor Biosynthesis, and Hydrolysis of Geranyl Pyrophosphate, the Acyclic Precursor of Camphor, by Enzymes from Sage (Salvia Officinalis)," Arch Biochem. Biophys. 198(2):523-532 (1979).
Gambliel, H., and Croteau, R., "Pinene Cyclases I and II," J. Biol. Chem. 259(2):740-748 (1984).
Croteau, R.B. et al., "Isotopically Sensitive Branching in the Formation of Cyclic Monoterpenes: Proof That (-)-.alpha.-Pinee and (-)-.beta.-Pinene Are Synthesized by the Same Monoterpene Cyclase via Deprotonation of a Common Intermediate," Biochemistry 26:5383-5389 (1987).
Croteau, R.B. et al., "Biosynthesis of Monoterpenes," J. Biol. Chem. 264(4):2075-2080 (1989).
Wagschal, K. et al., "Isotopically Sensitive Branching as a Tool for Evaluating Multiple Product Formation by Monoterpene Cyclases," Tetrahedron 47(31):5933-5944 (1991).
Wagschal, K.C. et al., "Monoterpene Biosynthesis: Isotope Effects Associated with Bicyclic Olefin Formation Catalyzed by Pinene Synthases from Sage (Salvia officinalis)," Arch. Biochem. Biophys. 308(2):477-487 (1994).
Croteau, R., "The Biosynthesis of Thujane Monoterpenes," In Recent Developments in Flavor and Fragrance Chemistry (Hopp, R., and Mori, K., Eds.), VCH Verlagsgesellschaft mbH, Weinheim, Germany, pp. 263-273 (1992).
Croteau, R., "Chapter 2. Monoterpene Biosynthesis. Cyclization of Geranyl Pyrophosphate to (+)-Sabinene," In Flavor Precursors: Thermal and Enzymatic Conversions (Teranishi, R., Takeoka, G.R., and Guntert, M., Eds.), American Chemical Society Symposium Series, Fourth Chemical Congress of North America (202nd National Meeting of the American Chemical Society), Washington, DC, No. 490, pp. 8-20, Aug. 25-30, 1991.
ColbyS.M. et al., "4S-Limonene Synthase from the Oil Glands of Spearmint (Mentha spicata)," J. Biol. Chem. 268(31):23016-23024 (1993).
Pyun, H.-J. et al., "Stereochemistry of the Proton Elimination in the Formation of (+)- and (-)-.alpha.-Pinene by Monoterpene Cyclases from Sage (Salvia officinalis), " Arch. Biochem. Biophys. 308(2):488-496 (1994).
Croteau, R. et al., "Biosynthesis of Monoterpenes: Partial Purification, Characterization, and Mechanism of Action of 1,8-Cineole Synthase," Arch. Biochem. Biophys. 309(1):184-192 (1994).
McGeady, P., and Croteau, R., "Isolation and Characterization of an Active-Site Peptide from a Monoterpene Cyclase Labeled with a Mechanism-Based Inhibitor," Arch Biochem. Biophys. 317(1):149-155 (1995).
Steele, C.L. et al., "Induced oleoresin biosynthesis in grand fir as a defense against bark beetles," Proc. Natl. Acad. Sci. USA 92:4164-4168 (1995).
Yuba, A. et al., "cDNA Cloning, Characterization, and Functional Expression of 4S-(-)-Limonene Synthase from Perilla frutescens," Arch. Biochem. Biophys. 332(21):280-287 (1996).
Bohlmann, J. et al., "Monoterpene Synthases from Grand Fir (Abies grandis)," J. Biol. Chem. 272(35):21784-21792 (1997).

Affiliated with

Also associated with

Rating

Monoterpene synthases from common sage (Salvia officinalis) does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Monoterpene synthases from common sage (Salvia officinalis), we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Monoterpene synthases from common sage (Salvia officinalis) will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-1370497