Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1992-09-30
1995-02-07
Saunders, David
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
435 74, 435 23, 436518, 436536, 436547, 424 9, 424 9467, C12Q 137, G01N 33536, G01N 33543, G01N 33573
Patent
active
053875044
ABSTRACT:
Monospecific antibodies are produced which are specific for fragments of the connective tissue protein aggrecan, generated by specific stromelysin cleavage. These monospecific antibodies are used in an assay system to detect polypeptide fragments of aggrecan, produced by the specific cleavage of aggrecan by stromelysin. The presence of aggrecan polypeptide fragments demonstrates stromelysin activity. Elevations of stromelysin occur in osteoarthritis, rheumatoid arthritis, atherosclerotic lesions, gout, inflammatory bowel disease (IBD), idiopathic pulmonary fibrosis (IPF), certain cancers, joint injuries, and numerous inflammatory diseases. The monospecific antibodies and the assay system are used to quantitate aggrecan polypeptide fragments as a readout of stromelysin activity and to evaluate potential stromelysin inhibitors.
REFERENCES:
patent: 4795702 (1989-01-01), Blake
Heinegard, et al., General Immunological 45, pp. 421-427 (1985).
Farndale, et al., Biochem. Biophys Acta 883; pp. 173-177 (1986).
Flannery, et al., J. Biol. Chem. 267; pp. 1008-1014 (1992).
Fosang, et al., J. Biol. Chem. 266; pp. 15579-15582 (1991).
Hascall and Kimura, Methods Enzymol 82 pp. 769-800 (1982).
Caterson, et al, Articular Cartilage Biochemistry, pp. 59-73 (1986).
Fosang et al, Sep. 25, 1992, The Interglobular Domain of Cartilage Aggrean is Cleaved by PUMP, Gelatinases, and Cathepsin B. J Biol. Chem. 267: 1948_70-74.
Hughes et al, Aug. 15, 1992, Monoclonal Antibodies Recognizing Protease-Generated Neoepitopes from Cartilage Proteoglycam Degradation. J Biol. Chem 267: 16011-14.
Hui et al, 1983. Monoclonal Antibodies to a Synthetic Fibrin-Like Peptide Bind to Human Fibrin but not Fibrinogen. Sci 222: 1129-32.
Doege et al, Jan. 15, 1991, Complete Coding Sequence and Deduced Primary Structure of the Human Cartilage Large Aggregating Proteoglycan, Aggrecan. J Biol. Chem 266: 894-902.
Hunter, 1979. "Radioimmunoassay", Handbook of Experimental Immunology. vol. 1. Immunochemistry, (D. M. Weir, Ed.) Blackwell Scientific Publications, Oxford. pp. 14.1-14.40.
Bayne Ellen B. K.
Hoerrner Lori A.
Lark Michael W.
Mumford Richard A.
Grun James L.
Matukaitis Paul D.
Merck & Co. , Inc.
Saunders David
Tribble Jack L.
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