Chemistry: molecular biology and microbiology – Spore forming or isolating process
Patent
1991-07-05
1992-09-15
Doll, John J.
Chemistry: molecular biology and microbiology
Spore forming or isolating process
4352402, 435 703, 435 704, 530399, 530827, C12N 500, C12N 508, C12P 2102, C07K 1506
Patent
active
051477980
ABSTRACT:
A continuous cell line of megakaryocytic origin is disclosed. This novel continuous cell line has been established in athymic nude mice. The cells of the established cell line monophenotypic for megakaryocytes is characterized as having multilobed nuclei and granular cytoplasm and being reactive for Factor VIII antigen, glycoprotein IIB-IIIa complex antigen and platelet peroxidase. The established cell line of this invention generates in isolatable quantities platelet-like factor IV, FGF-like, .beta.-thromboglobulin-like and TGF-.beta.-like growth factors. Methods for cultivating the established cell line, in a nude animal and isolating and purifying the proteins are also disclosed.
REFERENCES:
Tabilio et al., "Expression of Platelet Membrane Glycoproteins and .beta.-Granule Proteins by a Human Erythroleukemia Cell Line (HEL)", EMBO, v. 3(2), 453-459, 1984.
Morgan et al., "Novel Peripheral Blood-Derived Human Cell Lines with Properties of Megakaryocytes", J. Cell Biology v 100, 565-573, Feb. 85.
Pantazis et al., "Platelet-Derived Growth Factor Polypeptides in Human Megakryoblastic-Like Cell Lines" Cancer Cells 3, 153-157, 1985 ed:Feramiso et al..
Fieshey, "Introduction" in Culture of Animal Cells, pp. 1-6, 1983.
Harris Richard E.
Lampkin Beatrice C.
Liebermann Michael A.
Witte David P.
Children's Hospital Medical Center
Doll John J.
Elliott George C.
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