Monophenotypic in vitro cell lines of megakaryocytic lineage, pr

Details Monophenotypic in vitro cell lines of megakaryocytic lineage, pr Monophenotypic in vitro cell lines of megakaryocytic lineage, pr

1990-03-12

1993-03-30

Chan, Y. Christina

Chemistry: molecular biology and microbiology

Spore forming or isolating process

4352041, 43524023, C12N 500

Patent

active

051983567

ABSTRACT:
Human monophenotypic cloned cell lines of megakaryocytic lineage are established in vitro through use of adherent stromal cells in long term human bone marrow culture. Long term bone marrow cultures are used for initial adaptation of the cells to culture conditions. Once adapted, cells of the human in vitro cell lines are weaned from the stromal layer until they proliferate in the complete absence of any feeder layer. Seed cells for establishment of human in vitro cell lines were derived from a human solid tumor designated as ATCC CRL 9139 xenograft. Cells of one cloned in vitro cell line designated as CHRF-288-11 exhibits a karyotype and markers characteristic of megakaryocytes and platelets. Cells of the CHRF-288-11 cell line express platelet peroxidate, platelet factor IV, platelet Ca.sup.++ -ATPase, gpIIbIIIa, factor VIII, and MY7, MY9 and HLA-Dr antigens. CHRF-288-11 cell line exhibits a constant karyotype (50, XY). CHRF-288-11 cell line is deposited under the Budapest Treaty with American Type Culture Collection, 12301 Parklawn Drive, Rockville, Md. 20852, under accession No. CRL 10107.

REFERENCES:
Fugman, "Invitroestablishment and Characterization of a Human Megakaryocytic Cell Line CHRF-288 Utilizing Long-Term Bone Marrow Cultures as a Supportive Microenvironment", Thesis, University of Cincinnati, Mar. 1988.
Wyke et al., "Perturbed Hemopoiesis And the Generation of Multipotential Stem Cell Clones in SRC-infected Bone Marrow Cultures is an Indirect or Transient Effect of the Oncogene", Molecular and Cellular Biology, Mar. 1986, 959-963.
Tetteroo et al., "Megakaryoblastic Differentiation of Proetythroblastic 1562 Cell Line Cells", Leukemia Research, V. 8 (2), 197206, 1984.
Fugman et al., "In Vitro Establishment and Characterization of a Human Megakaryoblastic Cell Line", Blood, V. 75 (6), 1252-1261, 1990.

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