Monomethylamine-oxidizing enzyme

Details Monomethylamine-oxidizing enzyme Monomethylamine-oxidizing enzyme

1982-10-19

1985-03-05

Shapiro, Lionel M.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving oxidoreductase

435191, 435829, 435832, C12Q 126, C12N 906, C12R 105, C12R 107

Patent

active

045031479

ABSTRACT:
A monomethylamine-oxidizing enzyme can be obtained by cultivating in a medium a strain which belongs to Genus Bacillus and has an ability to produce a monomethylamine-oxidizing enzyme. This enzyme exhibits several beneficial properties including the ability to oxidatively deaminate the amino group of monomethylamine to produce formaldehyde, ammonia, and hydrogen peroxide. The enzyme exhibits a high substrate specificity for monomethylamine, ethylamine, and n-proplyamine while showing no substrate specificity for benzylamine, dimethylamine, trimethylamine, ethylenediamine and tryamine. In addition, the enzyme is stable through an elevated temperature range permitting faster reaction rates and therefore a shorter overall quantitative evaluation. Another property includes a low Km value which allows smaller quantities of the enzyme to be employed per sample.

REFERENCES:
Memoirs of the Research Institute for Food Science, vol. 27, pp. 1-13 (1966).

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