Monoclonal antibody which recognizes a specific glycoprotein of

Details Monoclonal antibody which recognizes a specific glycoprotein of Monoclonal antibody which recognizes a specific glycoprotein of

1989-04-05

1991-12-24

Kepplinger, Esther L.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

4351722, 43524027, 4365481, 530387, 935104, C12Q 100, G01N 3353, C12N 1500, C12N 500

Patent

active

050752197

ABSTRACT:
A hybridoma cell line is provided which is capable of producing a monoclonal antibody which binds to a unique determinant site on the surface and in the cytoplasm of human breast carcinoma cells and cells of other adenocarcinomas and does not bind selectively to the surface of normal breast epithelial cells except in instances of high concentration of the antibody in the testing fluid. The cell line of the invention was developed by immunizing mice with a select group of immunogens and a conventional myeloma cell line for fusion with the murine splenocytes harvested. The monoclonal antibody is identified as the BrE3 monoclonal antibody. The BrE3 monoclonal antibody binds to an antigen which is characterized as a high molecular weight glycoprotein complex having a molecular weight exceeding 400,000 daltons that is bound by a disulfide to a protein of 69,000 daltons. The BrE3 monoclonal antibody is especially useful for diagnostic detection, prognostic, and possible therapeutic application in human breast cell carcinoma.

REFERENCES:
Griffiths, A. et al., Int Jour Cancer 40:319-327 (1987).
Buchell, J. et al., Jour Immunol. 131(1):508-513 (1983).
Gendler, S., et al., PNAS 84:6060-6064 (1987).
Arklie, J. et al., Biol Abstr 73(1982) #26174.
van Hell, H. et al., In "Alternative Immunoassays", (Collins, ed., Wiley & Son, Publ.) 1985, pp. 39-58.
H. M. Geysen et al., Proc. Natl. Acad. Sci., 81: 3998-4002 (1984).

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