Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1995-07-07
1997-08-12
Housel, James C.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
435 792, 435189, 514 12, 554 83, G01N 3353
Patent
active
056564376
ABSTRACT:
A hybridoma cell line is described which produces and secretes a monoclonal antibody which specifically binds to vitellin in the eggs of the corn earworm, Helicoverpa zea, but does not bind to vitellin in the eggs of the tobacco budworm, Heliothis virescens. Eggs of H. zea may be detected and differentiated from eggs of H. virescens by subjecting a sample of insect eggs to an immunosorbent assay using the above-mentioned monoclonal antibody. The monoclonal antibodies may also be incorporated into kits for the detection of eggs of H. zea in the field.
REFERENCES:
Greenstone et al, Annals of the Entomalogical Soc. of Am. vol. 87(2) Mar. 1994, pp. 214-217.
Shaw, Cotton Pesticides Guide, 1993-1994, NSW Agriculture Austrialian Cotton Research Institute, Ed. by E. Roberts.
Forrester, Nell, et al., "Insecticide Resistance Management Strategy for Heliothis 1993-94", Cotton Pesticides Guide 1993-94, NSW Agriculture, Australian Cotton Research Institute, RMB Myall Vale, Narrabri, NSW 2390, pp. 8-13.
Greenston, Matthew H., "Bollworm or Budworm? Sqashbt Immunoassay Distinguishes Eggs of Helicoverpa Zea and Heliothis virescens (Lepidoptera: Noctuidae)", Journal of Economic Entomology, vol. 88, No. 2, Apr. 1995, pp. 213-218.
Greenstone, Matthew and Trowell, Stephen C., "Arthropod Prediation: A Simplified Immunodot Format for Predator Gut Analysis", Annals of the Entomological Soc. of America, vol. 87, No. 2, Mar. 1994, pp. 214-217.
Lenz, Cynthia L. and Greenstone, Matthew H., "Production of a Monoclonal Antibody to the Arylphorin of Heliothis zea", Archives of Insect Biochemistry and Physiology, 1988, pp. 167-177.
Deck Randall E.
Fado John D.
Housel James C.
Portner Ginny Allen
Silverstein M. Howard
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