Monoclonal antibody to thrombospondin and method for assaying fo

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...

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435 68, 4351722, 435240, 435948, 436548, 935103, 935110, 530387, G01N 3353, C12P 2100, A61K 39395, C12N 500

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046109609

ABSTRACT:
A monoclonal antibody produced by a hybridoma formed by the fusion of cells from a mouse myeloma line and spleen cells from a mouse previously immunized with thrombospondin. The monoclonal antibody reacts with human, bovine, and canine thrombospondin but not with the thrombospondin present in rabbit serum. The monoclonal antibody is capable of being used to identify human, bovine, and canine thrombospondin in ELISAs and by means of immunohistological techniques and also can be used to isolate such thrombospondin by when employed in immunoisolation techniques.

REFERENCES:
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Jaffe et al, Proc. Nat'l. Acad. Sci. USA, 80:998-1002 (Feb. 1983).
Jaffe et al, Nature, 295:246-248 (Jan. 21, 1982).
McClaren, J. Clin. Path., 36:197-199 (Feb. 1983).
Sage et al, Biochem., 22:2148-2155 (Apr. 1983).
Hagen et al, Biochem. Biophys. Acta, 732:600-606 (1983).

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