Monoclonal antibody to human lymphotoxin and use thereof

Details Monoclonal antibody to human lymphotoxin and use thereof Monoclonal antibody to human lymphotoxin and use thereof

1989-06-15

1993-02-23

Kepplinger, Esther L.

Chemistry: analytical and immunological testing

Involving production or treatment of antibody

Monoclonal antibody

43524027, 436518, 436824, 53038823, G01N 3353, C12N 520, C07K 1300

Patent

active

051889692

ABSTRACT:
A monoclonal antibody reactive with a C-terminal half of human lymphotoxin, and fragments thereof; a hybridoma cell line producing a monoclonal antibody reactive with a C-terminal half of human lymphotoxin; a process for the production of a monoclonal antibody reactive with a C-terminal half of human lymphotoxin, comprising the steps of culturing the above-mentioned hybridoma in a medium to secrete the antibody, and recovering the antibody from the culture supernatant; a process for the production of a monoclonal antibody reactive with a C-terminal half of human lymphotoxin, comprising the steps of inoculating a hybridoma to a mammal, obtaining ascites from the mammal, and recovering the monoclonal antibody from the ascites; a process for the production of a hybridoma cell line producing a monoclonal antibody reactive with a C-terminal half of human lymphotoxin, comprising the step of immunizing a mammal with a conjugate of zinc and a purified recombinant human lymphotoxin, obtaining spleen cells from the immunized mammal, fusing the spleen cells with myeloma cells, and cloning a hybridoma producing the monoclonal antibody; an adsorbent for lymphotoxin comprising a solid carrier and the above-mentioned monoclonal antibody or fragment of the antibody, wherein the monoclonal antibody is bonded to the surface of the solid carrier; a process for the purification of lymphotoxin comprising the steps of placing a material containing lymphotoxin in contact with the above-mentioned adsorbent to adsorb the lymphotoxin to the adsorbent, and eluting the adsorbed lymphotoxin with an eluent; and an assay method or assay kit for lymphotoxin using the above-mentioned monoclonal antibody or fragment thereof.

REFERENCES:
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patent: 4959457 (1990-09-01), Bringman
Eisen, in Immunology, Harper and Row, Inc., 1974, pp. 409-411.
T. S. Bringman et al. Hybridoma 6, 489(1987).
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P. W. Gray et al. Nature 312, 721(1984).
A. Meager et al. J. Immunol. Methods, 104, 31(1987).
Journal of Immunology, vol. 10, No. 1, 1989, pp. 93-105, Marcel Kekker, Inc.; H. Tada et al.
European Search Report.

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