Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1992-05-26
1994-10-11
Scheiner, Toni R.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
435 34, 435 38, 435 792, 43524027, 435849, 5303884, 530350, 530825, G01N 33569, G01N 33535
Patent
active
053546610
DESCRIPTION:
BRIEF SUMMARY
FIELD OF THE INVENTION
The present invention relates generally to hybridoma cell lines developed to produce monoclonal antibodies and, more specifically, to a cell line producing monoclonal antibodies to enterohemorrhagic Escherichia coli 0157:H7 (E. coli 0157:H7) and Escherichia coli 026:H11 (E. coli 026:H11).
DESCRIPTION OF THE PRIOR ART
E. coli 0157:H7 was first recognized as an important human pathogen in the United States in 1982, when the organism was diagnosed as the cause of two geographically separate outbreaks of hemorrhagic colitis, both associated with eating undercooked beef from a particular fast-food chain, (Riley, Lee W. et al, 1983, "Hemorrhagic Colitis Associated With A Rare Escherichia Coli Serotype," The New England Journal of Medicine, Vol. 308, No. 12, pgs. 681-685). These outbreaks of unusual gastrointestinal illness were characterized by the sudden onset of severe abdominal cramps and grossly bloody diarrhea with no fever or low grade fever. Such illnesses have been associated with E. coli 0157:H7 and also E. coli 026:H11 (Levine, M. M. 1987, "Escherichia coli that Cause Diarrhea: Enterotoxigenic, and Enteropathogenic, Enteroinvasive, Enterohemorrhagic, and Enteroadherent," Journal of Infectious Diseases, vol. 155, pgs 377-389; Levine, M. M , et al , 1987, "A DNA Probe to Identify Enterohemorrhagic Escherichia coli of 0157:H7 and Other Serotypes that Cause Hemmorhagic Colitis and Hemolytic Uremic Syndrome," Journal of Infectious Diseases, vol. 156, pgs. 175-182). These bacteria are termed enterohemorrhagic E. coli.
The E. coli organism produces toxins, known as verotoxins, that cause significant intestinal bleeding in several mammals, including humans. The spectrum of illnesses associated with E. coli 0157:H7 infections ranges from asymptomatic infections to non-bloody diarrhea, hemorrhagic colitis, hemolytic uremic syndrome and death (Ryan, Caroline A et al, 1986, "Escherichia coli 0157:H7 Diarrhea in a Nursing Home: Clinical, Epidemiological, and Pathological Findings," Journal of Infectious Diseases, Vol. 154, No. 4, pgs. 631-638). Hemolytic uremic syndrome is defined as the sudden onset of hemolytic anemia, thrombocytopenia and acute renal failure after the appearance of symptoms in the upper respiratory tract, stomach or intestines. Hemolytic uremic syndrome is generally the end result of a number of different and inciting events and pathogenic mechanisms.
The organism has been isolated from meat and poultry and unpasteurized milk. A variety of reports suggest that foods, particularly foods of animal origin, may be an important source of E. coli 0157:H7 infections (Doyle, Michael P. and Jean L. Schoeni, 1987, "Isolation of "Escherichia Coli 0157:H7 from Retail Fresh Meats and Poultry," Applied and Environmental Microbiology, vol. 53, No. 10, pgs. 2394-2396).
Because most of the outbreaks of hemorrhagic colitis have been food related, there is a need for a rapid, sensitive and specific assay for detecting E. coli 0157:H7 and E. coli 026:H11. Presently, the available methods for detecting the organism in foods are time consuming or are not highly specific. For example, the Food Research Institute, Madison, Wis., is currently being used by many agencies for the detection of the E. coli 0157:H7 organism. The process utilized by the Food Research Institute, however, involves a complicated, multi-day procedure described in Doyle and Schoeni (supra). Doyle and Schoeni have tested a variety of meats and found the organism in about 1% or 2% of the meats tested. The organism can also be isolated from feces, raw milk, etc. Eggs from experimentally infected chickens are also contaminated on the surface.
In order to assay the organism, the first step is to enrich the organism in the food sample. Because the food sample may have minor amounts of the organism, the sample is placed in an enrichment media to put it in a condition which should repress other forms of bacteria and increase the numbers of the desired organism. The enrichment medium has selective agents which are selective for g
REFERENCES:
patent: 5100801 (1992-03-01), Ward et al.
patent: 5168063 (1992-12-01), Doyle et al.
Siragusa, G. R., et al., "Persistence of Listeria-monocytogenes in Yogurt as Determined by Direct Plating and Enrichment Methods," Int. J. Food Microbiol. 7, pp. 147-160, 1988, Abstract only.
Doyle, M. P., et al., "Isolation of Escherichia coli 0157:H7 from Retail Fresh Meats and Poultry," Applied and Environmental Microbiology, vol. 53, No. 10, pp. 2394-2396, Oct. 1987.
Bopp, C. A., et al., 1987, "Unusual Verotoxin-Producing Escherichia coli Associated with Hemorrhagic Colitis," J. Clin. Microbiol., vol. 25, pp. 1486-1489.
Hancock, R. E. W. and H. Naikaido, 1987, "Outer Membranes of Gram-Negative Bacteria," Journal of Bacteriology, vol. 136, pp. 381-390 (isolating of outer membrane proteins).
Karmali, M. A. et al., 1983, "Sporadic Cases of Hemolytic Uremic Syndrome Associated with Fecal Cytoxin and Cytoxin Producing Escherichia coli in Stools," Lancet, vol. i, pp. 619-620.
Levine, M. M., 1987, "Escherichia coli that Cause Diarrhea: Enterotoxigenic, Enteropathogenic, Enteroinvasive, Enterohemorrhagic, and Enteroadherent," Journal of Infectious Diseases, vol. 155, pp. 377-389.
Levine, M. M., et al., 1987, "A DNA Probe to Identify Enterohemorrhagic Escherichia coli of 0157:H7 and Other Serotypes that Cause Hemmorhagic Colitis and Hemolytic Uremic Syndrome," Journal of Infectious Diseases, vol. 156, pp. 175-182.
Padhye, N. P. and M. P. Doyle, 1990, "Production and Characterization of Monoclonal Antibody to Escherichia coli 0157:H7", Amer. Soc. for Microb. (Annual Mtg.) (Abstract), P-14, p. 280.
Padye, V. V., et al., 1990 "Production and Characterization of Monoclonal Antibodies to Verotoxins 1 and 2 from Escherichia coli of Serotype 0157:H7," Biological Abstracts, vol. 89, No. 5, the abstract No. 48707.
Padhye, V. V. et al., 1989, "Production and Characterization of Monoclonal Antibodies to Verotoxins 1 and 2 from Escherichia coli of Serotype 0157:H7," J. Med. Microb., vol. 30, pp. 219-226.
Perera, L. P., et al., 1988, "Isolation and Characterization of Monoclonal Antibodies to Shiga-Like Toxin II of Enterohemorrhagic Escherichia coli and Use of the Monoclonal Antibodies in a Colony Enzyme-Linked Immunosorbent Assay," Journal of Clinical Microbiology, vol. 26, No. 10, pp. 2127-2131, see the Abstract.
Perry, M. B. et al., 1988, "Identification of Escherichia coli Serotype 0157 Strains by Using a Monoclonal Antibody," J. Clin. Microbiol., vol. 26, pp. 1973-1978.
Pollard, D. R. et al., 1990, "Rapid and Specific Detection of Verotoxin Genes in Escherichia coli by the Polymerase Chain Reaction", J. Clin. Microb., vol. 28, pp. 540-545.
Riley, Lee W. et al, 1983, "Hemorrhagic Colitis Associated With A Rare Escherichia Coli Serotype," The New England Journal of Medicine, vol. 308, No. 12, pp. 681-685.
Ryan, Caroline A. et al, 1986, "Escherichia coli 0157:H7 Diarrhea in a Nursing Home: Clinical, Epidemiological, and Pathological Findings," Journal of Infectious Diseases, vol. 154, No. 4, pp. 631-638.
Samadpour, M. et al., 1990, "Evaluation of DNA probes for detection of Shiga-Like-Toxin Producing Escherichia coli in Food and Calf Fecal Samples," Appl. and Envir. Microb., vol. 56, pp. 1212-1215.
Smith et al., 1985, "Measurement of Protein Using Bicinchoninic Acid," Analytical Biochemistry, vol. 150, pp. 76-85 (determination of protein concentration).
Todd, E. C. D. et al, 1988, "Rapid Hydrophobic Membrane Filter-Enzyme Labeled Antibody Procedure for Identification and Enumeration of Escherichia coli 0157:H7 in Foods" Applied and Environmental Microbiology, vol. 54, pp. 2536-2540.
Tzipori, S., et al., 1988, "Studies in Gnotobiotic Piglets on Non-0157:H7 Escherichia coli Serotypes Isolated from Patients with Hemorrhagic Colitis," Gastroenterology, vol. 94, No. 9, pp. 590-597, see the abstract.
Doyle Michael P.
Padhye Nisha
Scheiner Toni R.
Wisconsin Alumni Research Foundation
LandOfFree
Monoclonal antibody to enterohemorrhagic Escherichia coli 0157:H does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Monoclonal antibody to enterohemorrhagic Escherichia coli 0157:H, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Monoclonal antibody to enterohemorrhagic Escherichia coli 0157:H will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1658011