Monoclonal antibody to CD166 and method for production thereof

Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Blood proteins or globulins – e.g. – proteoglycans – platelet...

Reexamination Certificate

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C435S004000, C435S007100, C530S388100

Reexamination Certificate

active

08003762

ABSTRACT:
CD166 is a cell adhesion molecule belonging to an immunoglobulin superfamily that is expressed in an excessive amount on the tumor surface. If an monoclonal antibody specifically binding to the CD166 is obtained, it becomes possible to suppress growth of tumor cells, detect the cells, and supply a therapeutic drug thereto specifically. However, because the CD166 proteins are very similar to each other among mammals, it was not possible to obtain an antibody to human CD166, by immunizing, for example, mice with the human CD166.The antibody was prepared by immunizing mice with a purified avian CD166 protein. The antibody was found to be adsorbed on human and mouse CD166 proteins in vitro as well as in vivo and to have an action to suppress tumor growth in mice.

REFERENCES:
patent: 5998172 (1999-12-01), Haynes et al.
Piazza et al, J Cell Sci. vol. 118, pp. 1515-1525, Apr. 2005.
Kempen et al , JBC 276:25783-90, 2001.
Piazza et al, J of Cell Science, vol. 118, p. 1515-1525, published online Mar. 2005.
Bowen et al , Eur J Immuno 27:01469-1478, 1997.
Sequence search result (Hynes), 2010.
Mesh word search result (CD166), 2010.

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