Monoclonal antibody to angiotension-converting enzyme and method

Details Monoclonal antibody to angiotension-converting enzyme and method Monoclonal antibody to angiotension-converting enzyme and method

Filed on

1983-02-15

Date issued

1986-04-15

Examiner

Rosen, Sam

Patent Class

Chemistry: molecular biology and microbiology

SubClass

Measuring or testing process involving enzymes or...

SubSubClass

Involving antigen-antibody binding, specific binding protein...

Other Related Categories

435 68, 4351722, 435240, G01N 33535, C12N 1500

Type

Patent

Status

active

Patent number

045827907

Description

ABSTRACT:
A method for preparing a monoclonal antibody of improved cross-species specificity. The method includes the steps of preparing an inoculum antigen from a first species of animal producing the antigen and then immunizing a selected subject animal with the inoculum antigen. The method further includes the steps of fusing spleen cells from the immunized subject animal with myeloma cells to produce hybridomas. A test antigen is prepared corresponding to the inoculum antigen but derived from a second species of animal, distinct from the first species of animal from which the inoculum antigen was prepared. Hybridomas are screened for desired antibody production against the test antigen. The hybridoma producing antibodies most active against the test antigen is then cultured, and monoclonal antibodies are produced therefrom.

REFERENCES:
patent: 4196265 (1980-04-01), Koprowski et al.
patent: 4381295 (1983-04-01), Kung et al.
patent: 4487829 (1984-12-01), Sharp et al.
Auerbach et al. -Microvascular Research, vol. 29 (1985) pp. 401-411.
Auerbach et al., -Proc. Nat. Acad. Sci.; vol. 79 (Dec. 1982) pp. 7891-7895.

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