Monoclonal antibody that specifically binds to disialosyl Le.sup

Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Blood proteins or globulins – e.g. – proteoglycans – platelet...

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530389, 43524027, 435 7, 4351722, 424 11, 424 8591, 424 858, 935104, 935110, 436548, C07K 1500, C12N 500, G01N 5300, A61K 4300, A61K 3900

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048515116

ABSTRACT:
Disialosyl Le.sup.a (IV.sup.3 NeuAcIII.sup.6 NeuAcIII.sup.4 FucLc.sub.4), a novel human cancer-associated fucoganglioside that is highly immunogenic. Also, a hybridoma cell line (ATCC No. HB 8861)secreting a monoclonal IgG3 antibody (FH7) directed to disialosyl Le.sup.a. The disialosyl Le.sup.a antigen was detected in various cancer tissues and their cell lines but was absent in various normal tissues and blood cells. Sera of patients with various cancers, particularly early cases of colonic and gastric cancers, showed an elevated disialosyl Le.sup.a antigen level, which subsequently decreased after surgical tumor removal.

REFERENCES:
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Fukushi, Y. et al., "Novel Fucolipids Accumulating in Human Adenocarcinoma. III. A Hybridoma Antibody (FH6) Defining a Human Cancer-Associated Di-fucoganglioside VI.sup.3 Neu AcV.sup.3 III.sup.3 Fuc.sub.2 nLc.sub.6." Journal of Biological Chemistry 259(16): 10511-10517, Aug. 25, 1984.
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