Monoclonal antibody specific to the lymphokine LK 2 and its meth

Details Monoclonal antibody specific to the lymphokine LK 2 and its meth Monoclonal antibody specific to the lymphokine LK 2 and its meth

1988-07-21

1991-07-09

Nucker, Christine

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Using tissue cell culture to make a protein or polypeptide

4351722, 43524027, 530351, 530387, 935 93, C12P 2108

Patent

active

050305646

ABSTRACT:
A monoclonal antibody specific to the lymphokine LK 2, and its production is disclosed. The novel lymphokine LK 2 is a glycoprotein with a molecular weight of 20,000.+-.2,000 daltons; isoelectric point pI, 6.2.+-.0.3; electrophoretic mobility Rf, 0.29.+-.0.02; cytotoxic on L 929 cells; and not substantially growth inhibitive on KB cells. The lymphokine significantly inhibits the growth of malignant human tumors in vivo. The monoclonal antibody may be of IgM or IgG class, and neutralizes specifically the cytotoxic activity of the lymphokine. Combined use of LK 2 with chemotherapeutic agents such as alkylating agents, metabolic antagonists, antioncotic antibiotics and plant alkaloids enhances greatly the antioncotic effect of the chemotherapeutics.

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Warren, H. S. et al., "A Method for the Production and Quantitative Assay of Human Lymphokine Preparations", Biol Abstr, vol. 72, No. 5, 1981, p. 3282, ref. nr. 31342.
Ruff, M. R., et. al. "Tumor Necrosis Factor", in Lymphokines, vol. 2, edited by E. Pick, Academic press, New York, NY, 1981, pp. 235-272.

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