Monoclonal antibody specific to tartrate-resistant acid...

Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Blood proteins or globulins – e.g. – proteoglycans – platelet...

Reexamination Certificate

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C530S391100, C435S007400, C435S007920, C435S007940, C435S007950, C435S021000, C435S070210, C435S040520, C435S174000, C435S177000, C435S452000, C435S338000, C435S960000, C435S975000, C436S518000, C436S528000, C436S548000

Reexamination Certificate

active

07074903

ABSTRACT:
Monocolonal antibodies having a higher reactivity with tartrate-resistant acid phosphatase 5b (TRACP 5b) than tartrate-resistant acid phosphatase 5a (TRACP 5a) and having a higher specificity to TRACP 5b can be obtained by cell fusion using as antigens TRACP 5b purified from human osteoclasts. By using the monoclonal antibody, TRACP 5b in a sample can be detected specifically with a high sensitivity.

REFERENCES:
patent: 6248544 (2001-06-01), Halleen et al.
patent: WO 99/50662 (1999-10-01), None
T. Onishi; “Study of Human Serum Tartrate-Resistant Acid Phosphatase”;J. Nihon University Medical Association;vol. 49, No. 9; 1990./English abstract on p. 911/Discussed in the specification .
K.-H. Lau et al.; “Characterization and Assay of Tartrate-Resistant Acid Phosphatase Activity in Serum: Potential Use to Assess Bone Resorption”;Clinical Chemistry;vol. 33, No. 4; pp. 458-462; 1987./Discussed in the specification.
A. Janckila et al.; “Tartrate-resistant Acid Phosphatase Isoform 5b as Serum Marker for Osteoclastic Activity”;Clinical Chemistry;vol. 47, No. 1; pp. 74-80; 2001.
M. Kraenzlin et al.; “Development of an Immunoassay for Human Serum Osteoclastic Tartrate-Resistant Acid Phosphatase”;Journal of Clinical Endocrinology and Metabolism;vol. 71, No. 2; pp. 442-451;1990./ Discussed in the specification.
J. Haleen et al.; “Two-Site Immunoassays for Osteoclastic Tartrate-Resistant Acid Phosphatase Based on Characterization of Six Monoclonal Antibodies”;Journal of Bone and Mineral Research;vol. 14., No. 3; pp. 464-469; 1999./Discussed in the specification.
H. Bull et al.; “Reactivity and assay restriction profiles of monconal and polyclonal antibodies to acid phosphatases: a preliminary study”;Immunology Letters;vol. 70; pp. 143-149; 1999./Discussed in the specification.
S. Alatalo et al.; “Rapid Screening Method for Osteoclast Differentiation in Vitro That Measures Tartrate-resistant Acid Phosphatase 5b Activity Secreted into the Culture Medium”;Clinical Chemistry;vol. 46., No. 11; pp. 1751-1754; 2000./Discussed in the specification.
J. Halleen et al.; “Characterization of Serum Tartrate-Resistant Acid Phosphatase and Development of a Direct Two-Site Immunoassay”;Journal of Bone and Mineral Research;vol. 13, No. 4; pp. 683-687; 1998./Discussed in the specification.
Y. Nakasato et al.; “Clinical Significance of Immunoassays for Type-5 Tartrate-resistant Acid Phosphatase”;Clinical Chemistry;vol. 45; No. 12; pp. 2150-2157; 1999./Discussed in the specification.
K. Takahashi et al.; “Electrophoretic study of tartrate-resistant acid phosphatase isoforms in endstage renal disease and rheumatoid arthritis”;Clinica Chimica Acta;vol. 301; pp. 147-158; 2000./Discussed in the specification.
W. Lam et al.; “Biochemical Properties of Tartrate-Resistant Acid Phosphatase in Serum of Adults and Children”;Clinical Chemistry;vol. 24, No. 7; pp. 1105-1108; 1978.
W. Lam et al.; “Tartrate-Resistant (Band 5) Acid Phosphatase Activity Measured by Electrophoresis on Acrylamide Gel”;Clinical Chemistry;vol. 24., No. 2; pp. 309-312; 1978.
M. Nakanishi et al.; “Development of a Kinetic Assay for Band 5B Tartrate-resistant Acid Phosphatase Activity in Serum”;Clinical Chemistry;vol. 46; No. 4; pp. 469-473; 2000.
A. Hayman et al.; “Purple Acid Phosphatase of the Human Macrophage and Osteoclast”;The Journal of Biological Chemistry;vol. 269, No. 2; pp. 1294-1300; 1994.
A. Janckila et al.; “Characterization of Monoclonal Antiboides Specific to Human Tartrate-Resistant Acid Phosphatase”;Hybridoma;vol. 16, No. 2; pp. 175-182; 1997.
A. Janckila et al.; “Species Specificity of Monoclonal Antibodies to Human Tartrate-Resistant Acid Phosphatase”;Biotechnic and Histochemistry;vol. 73; No., 6; pp. 316-324; 1998.
M. Nakanishi et al.; “Improved method for measuring tartrate-resistant acid phosphatase activity in serum”;Clinical Chemistry;vol. 44, No. 2; pp. 221-225; 1998.
Copy of Communication and European Search Report dated Dec. 4, 2003.
J. Hallen et al., Journal of Bone and Mineral Research, “Tartrate-Resistant Acid Phosphatase 5b: A Novel Serum Marker of Bone Resorption”, vol. 15, No. 7, Jul. 2000, pp. 1337-1345.

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