Monoclonal antibody specific to nitrifying bacteria and method f

Details Monoclonal antibody specific to nitrifying bacteria and method f Monoclonal antibody specific to nitrifying bacteria and method f

1996-10-30

1998-06-30

Caputa, Anthony C.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving antigen-antibody binding, specific binding protein...

435 71, 435326, 435332, 435340, 5303882, 5303884, G01N 3353, G01N 33554, C07K 1600, C12P 2108

Patent

active

057732337

ABSTRACT:
The present invention relates to a monoclonal antibody showing a high specificity to Nitrosomonas europaea or Nitrobacter agilis, and a monoclonal antibody-sensitized latex for detecting Nitrosomonas europaea or Nitrobacter agilis used for facilitating the detection of nitrifying bacteria present in activated sludge, water, soil or microorganism-immobilized carriers, and besides a method of detecting nitrifying bacteria using said monoclonal antibody-sensitized latex. The present invention provides a monoclonal antibody to nitrifying bacteria having ammonia oxidation activities or nitrite oxidation activities present in activated sludge or soil, or microorganism-immobilized carriers. This monoclonal antibody to Nitrosomonas europaea or Nitrobacter agilis is prepared by culturing clones of fused cells (hybridomas) obtained by fusing antibody-forming cells and tumor cells obtained from an animal immunized with Nitrosomonas europaea or Nitrobacter agilis.

REFERENCES:
Sevier et al (Clinical Chemistry vol. 27 No. 11 pp. 1797-1806), 1981.

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