Chemistry: molecular biology and microbiology – Spore forming or isolating process
Patent
1987-11-06
1989-12-19
Nucker, Christine M.
Chemistry: molecular biology and microbiology
Spore forming or isolating process
435 5, 435 7021, 4351722, 540387, 540808, 540809, 935110, C12N 1500, G01N 33567, G01N 33577
Patent
active
048882909
ABSTRACT:
A hybrid cell line is provided which is capable of producing monoclonal antibodies which bind to HIV core antigen P55, the precursor protein coded for the gag gene, the core protein P24 and partial breakdown products P39 and P33. The monoclonal antibody embodying the invention does not bind the P18 core protein or any HIV envelope antigens. The cell line of the invention was developed by a unique immunization protocol in which BALB/c mice were immunized over a series of multiple infusions using a select group of immunogens and a conventional myeloma cell line for fusion with the murine splenocytes harvested.
The monoclonal antibody is identified as the KC-57 antibody. This monoclonal antibody is especially useful for a solid phase immunoassay in which the monoclonal antibody is detected in its binding to HIV antigens found in a serum or plasma sample from a human patient. The cell line which produces the KC-57 monoclonal antibody has been deposited in the American Type Culture Collection, Rockville, Md. and assigned A.T.C.C. No. HB 9585.
REFERENCES:
Hattori et al., "Characterization of Three Monoclonal Antibodies (VAK3-5) That Identify p24, Core Protein of Human Immunodeficiency Virus, and its Precursors", Jpn. J. Cancer Res. (Gann), 78 (1987) 235-241.
Caruso et al., "Liquid Competition Radioimmunoassay for the Detection and Quantitation of the HIV p24", J. Virol. Meth., 17 (1987) 199-210.
Higgins et al., "Detection and Differentiation by Sandwich Enzyme-Linked Immunosorbent Assay of Human T-Cell Lymphotropic Virus Type III/Lymphadenopathy-Associated Virus-and Acquired Immunodeficiency Syndrome-Associated Retroviruslike Clinical Isolates" J. Clin. Microbiol., 24 (1986) 424-430.
Kanki et al., "Serologic Identification and Characterization of a Macaque T-Lymphotropic Retrovirus Closely Related to HTLV-III", Science, 228 (1985) 1199-1201.
Ferns et al., "Characterization of Monoclonal Antibodies Against the Human Immunodeficiency Virus (HIV) gag Products and Their Use in Monitoring HIV Isolat Variation", J. Gen. Virol., 68 (1987) 1543-1551.
Hofheinz David E.
Kortright Kenneth H.
Sullivan Carole
Toedter Gary P.
Cass Myron C.
Coulter Corporation
Nucker Christine M.
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