Monoclonal antibody specific for antigens in mouse inbred strain

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...

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435960, 436519, 5303882, 53038885, G01N 33573

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active

055522874

ABSTRACT:
A novel monoclonal antibody of IgG1/.kappa. subclass, characterized in that it recognizes antigens specifically present in mouse inbred strains consisting of DBA/1, CE/J, SM/J, IQI, PL/J, SWM/Ms, RIIIS/J, RFM/MsNrs and C3H and its congenic mouse strains thereof, wherein one of said antigens has a molecular weight of 66,000 and the other has a molecular weight of 68,000 and said antigens are present in the mitochondria of C3H mouse strain.
The present invention also provides a hybridoma cell line which produces the above monoclonal antibody, a method for identifying the origin of a cell derived from a mouse, and a reagent for identifying the origin of a cell derived from a mouse.

REFERENCES:
Kusakabe, et al. J. Cell Biol., 107, 257-265 (1988).
Kusakabe et al., 1988, A novel methodology for analysis of all distribution in chimetic mouse organs using a strain specific antibody. J Cell Biol. 107: 257-265.
Lee et al., Jun. 1991, Strain specific sensitivity to diethylmitrosamine-induced carcinogenesis is maintained in chepatocytes of C3H/HeN.rarw..fwdarw.C57BL/6N chimeric mice, Cancer Res 51: 3257-3260.
Goding, 1983. Monoclonal Antibodies: Principles and Practice.
Production and Application of Monoclonal Antibodies in Cell Biology, Biochemistry and Immunology. Academic Press, Orlando, pp. 8, 40, 56-97.
Mosmann et al., 1989. TH1 and TH2 cells: different patterns of lymphokine secretion lead to different functional properties. Ann. Rev Immunol. 7: 145-173.

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