Monoclonal antibody purification process using protein A

Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Blood proteins or globulins – e.g. – proteoglycans – platelet...

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424101, 424 858, 514 8, 514 21, 530809, 530830, A61K 39395

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active

048016870

ABSTRACT:
Immunoglobins are purified by adsorption upon an immobilized protein A adsorbent using a buffer having a pH of 7.5 to 10 and containing a combination of monovalent cations and polybasic anions in a concentration of about 0.6M to 1.75M.

REFERENCES:
patent: 3850798 (1974-11-01), Sjoquist
patent: 4582875 (1986-04-01), Ngo
Febs Letters, vol.-28 (1972), 73-76, Hztelm et al.
Immunochemistry, 15 (1978), 429-436, Ey et al.
Journal of Immunological Methods 62, (1983), 1-13, Lindmark et al.
Bulletin 1172, Bio-Rad Laboratories, Bio-Rad Chemical Division, Richmond,. Calif., (1984).

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