Monoclonal antibody, method of production thereof and use thereo

Details Monoclonal antibody, method of production thereof and use thereo Monoclonal antibody, method of production thereof and use thereo

1992-06-25

1994-03-01

Rosen, Sam

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving antigen-antibody binding, specific binding protein...

435 7021, 4351722, 43524027, 436548, 53038824, C12Q 100, G01N 33535

Patent

active

052906809

ABSTRACT:
The present invention relates to a monoclonal antibody which specifically recognizes 6,15-diketo-13,14-dihydro-prostaglandin F.sub.1 .alpha. with high sensitivity, a hybridoma producing said monoclonal antibody, a method of establishing said hybridoma and an immunoassay method for 6,15-diketo-13,14-dihydro-prostaglandin F.sub.1 .alpha. using said monoclonal antibody.
The monoclonal antibody of the present invention makes it possible to conveniently measure 6,15-diketo-13,14-dihydro-prostaglandin F.sub.1 .alpha., a stable metabolite of prostaglandin I.sub.2 thought of as reflecting the concentration changes therein, with specificity and high sensitivity. It is useful in determining the content of prostaglandin I.sub.2, which exhibits platelet aggregating inhibitory activity, vasodilating activity and other activities, in biological samples.

REFERENCES:
A. Edlund et al., "Cardiac Formation of Prostacyclin During Cardioplegia in Man", Prostaglandins, vol. 24, No. 1, Jul. 1982, pp. 5-19.
C. Machleidt et al., "Formation and Elimination of Proostacyclin Metabolites in the Cat in vivo as Determined by Radioimmunoassay of Unextracted Plasma", Chem. Abs., vol. 95, No. 21, Nov. 23, 1981.
Machleidt et al--European J. of Pharmacol, vol. 74 (1981) pp. 19-26.
Forder et al.--Chem. Abst., vol. 100 (1984), p. 29785d.
Gruetzmann et al.--Chem. Abst., vol. 100 (1984), p. 62512k.
Brune et al.--Chem. Abst., vol. 103 (1985), p. 65097b.

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