Monoclonal antibody for recovery of leukocytes in human peripher

Details Monoclonal antibody for recovery of leukocytes in human peripher Monoclonal antibody for recovery of leukocytes in human peripher

1985-11-19

1988-06-21

Moskowitz, Margaret

Chemistry: molecular biology and microbiology

Maintaining blood or sperm in a physiologically active state...

435 4, 435 7, 435 29, 435 68, 435 70, 4351722, 43524027, 436518, 436526, 436528, 436534, 436548, 530387, 935108, C12Q 102, G01N 33577

Patent

active

047525630

ABSTRACT:
A murine monoclonal antibody which selectively binds to the determinant site or specific epitope of glycophorin A exposed on the membrane of the erythrocyte and does not bind to other glycophorins. The monoclonal antibody is coated on a microsphere or substrate of a suitable monodispersed variety and utilized in a separation procedure for recovery of white blood cell subsets without lysing of erythrocytes. The bound microspheres are recovered without adverse depletion of the white blood cell population of the sample. The microsphere or bead-to-cell ratio employed is most acceptable for commercial applications of the invention. The invention thus enables accurate assaying of white blood cell subsets in circulating peripheral blood without resorting to lysing of erythrocytes.

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