Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Reexamination Certificate
1997-10-02
2001-06-26
Scheiner, Laurie (Department: 1641)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
C435S007920, C530S388100, C530S388900, C530S389800
Reexamination Certificate
active
06251612
ABSTRACT:
FIELD OF THE INVENTION
The present invention relates to a monoclonal antibody for an immunoassay of detergents and their degradation products, and its use. More specifically, it relates to a hybridoma which produces the monoclonal antibody, the monoclonal antibody produced by the hybridoma, and a kit and a method for immunoassay of detergents, their degradation products or a mixture thereof.
BACKGROUND OF THE INVENTION
Among surfactants for synthetic detergents, a linear alkylbenzenesulfonate (hereinafter sometimes abbreviated to LAS), which is an anionic surfactant, has been sold and used since 1967 instead of a branched alkylbenzenesulfonate (ABS), which has poor biodegradability, according to the recommendation of the administrative. Since LAS is cheap and its toxicity is low, it is still using as a main component of household synthetic detergents. However, recently, water pollution by LAS and its degradation products have been a social problem because of the discharge of drainage from living into rivers, etc. Then, the tap water standard of anionic surfactants has been established (not more than 0.2 mg/liter). In addition, nonionic surfactants are used as about 40% of surfactant compounds for synthetic detergents and establishment of its tap water standard is being studied.
Under these circumstances, synthetic detergents and their degradation products in an environment are determined and analyzed and the results are utilized for environmental preservation. As methods for such determination and analysis, for example, high-performance liquid chromatography (HPLC) and gas chromatography (GC) are employed for quantitative determination of detergents and their degradation products in tap water, rivers, swamps, lakes or sewege etc. However, the problems of these methods are such that they require expensive apparatus or devices, pretreatment and the operation with great skill. As official methods for measuring anionic surfactants and nonionic surfactants, there are methylene blue absorption method and potassium zinc (II) tetrathiocyanate method, respectively. However, these methods require, for example, extraction with organic solvents which are harmful to human beings and complicated concentration steps and therefore, improvement of these methods is expected.
In view of these problems, regarding anionic and nonionic surfactants, it is desired to develop a method for determination thereof which can maintain present high sensitivity and can be readily and quickly operated with high specificity at a low cost.
As one means for solving these problems, an enzyme-linked immunosorbent assay (hereinafter sometimes abbreviated to ELISA) can be considered.
Assay systems and kits utilizing ELISA have been constructed for various other environmental pollutants. For example, WO 94/12536 discloses ELISA for detecting petroleum fuel in an environment and a kit for such ELISA. The kit comprises an enzyme and an antibody, and the measurement can be completed very quickly, usually, within several hours. And, the operation is very simple in comparison with conventional HPLC and GC, and, in most cases, no complicated pretreatment required in such conventional methods is needed. Further, a very specific determination can be carried out by using an antibody, in particular, a monoclonal antibody. HPLC and GC require very expensive apparatus and devices and their maintenance is also expensive, while ELISA has no such problem.
However, any immunoassay method which can be used for detection and determination of synthetic detergents including LAS, in particular, ELISA has not yet been established.
OBJECTS OF THE INVENTION
In order to establish an immunoassay method for detection and determination of synthetic detergents, one object of the present invention is to provide a hybridoma which can produce a monoclonal antibody useful for an immunoassay of synthetic detergents, their degradation products and a mixture thereof.
Another object of the present invention is to provide the monoclonal antibody produced by the hybridoma of the present invention.
Another object of the present invention is to provide a kit for an immunoassay of synthetic detergents, their degradation products and a mixture thereof.
Still another object of the present invention is to provide a method for immunoassay of synthetic detergents, their degradation products and a mixture thereof.
These objects as well as other objects and advantages of the present invention will become apparent to a person skilled in the art from the following description with reference to the accompanying drawings.
REFERENCES:
patent: 92/15014 (1992-09-01), None
patent: 94/12536 (1994-06-01), None
Wong et al., J. Lipid Res. 26 (5). 1985, 528-539.
Fujimoto Shigeru
Fujita Masanori
Goda Yasuhiro
Miyagawa Ken-ichiro
Toyoda Yukio
Foley & Lardner
Scheiner Laurie
Takeda Chemical Industries Ltd.
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