Monoclonal antibody detection of proteins on sperm related to fe

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...

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435 71, 435 4, 530350, G01N 3353

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059622415

ABSTRACT:
Heparin binding proteins (HBP) are produced by male accessory glands and bind to sperm at ejaculation. The ability of accessory glands to produce HBP and sperm to bind HBP differs among males. Identifying the presence of a 21.5, 24 and 30 kDa heparin binding proteins in sperm membranes with a monoclonal antibody resulted in separating groups of bulls by 40% difference in fertility. Thus, fertility potential of a bull can be predicted by heparin binding protein content of sperm membranes.

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