Monoclonal antibody-based immunoassay for cyclic DNA adducts res

Details Monoclonal antibody-based immunoassay for cyclic DNA adducts res Monoclonal antibody-based immunoassay for cyclic DNA adducts res

1988-01-15

1990-05-08

Warden, Robert J.

Chemistry: molecular biology and microbiology

Treatment of micro-organisms or enzymes with electrical or...

Modification of viruses

43524027, 435948, 436548, 436813, 436822, 530387, 530809, 935104, 935110, G01N 33577

Patent

active

049238132

ABSTRACT:
Monoclonal antibodies specific for the 8R,6R- and 8S,6S-stereoisomers of 3-(2-deoxy-.beta.-D-erythropentofuransyl)-5,6,7,8-tetrahydro-8-hydroxy-6-m ethylpyrimido[1,2-a]purine-10(3H)one were produced. These cyclic 1,N.sup.2 -propanodeoxyguanosines are formed in DNA exposed to crotonaldehyde in vitro. Three of the four antibodies were most specific for one stereoisomer while the fourth was most specific for the other stereoisomer. Fifty % inhibition of binding in an enzyme-linked immunoabsorbent assay could be achieved with 0.2 picomol of either stereoisomer. A high-pressure liquid chromatography-enzyme-linked immunoabsorbent assay using two of these antibodies and capable of detecting 0.5 .mu.mol of 1,N.sup.2 -propanodeoxyguanosine per mol of deoxyguanosine was developed. The method was validated by comparison to results obtained with fluorescence assay.

REFERENCES:
patent: 4794074 (1988-12-01), Harris
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Erlanger, Meth. Enzymol., 70, 88-89, 1980.
Foiles et al, Chem. Abstr., 106, Abstr. No. 133152e, 1987.
Hertzog et al, Journ. Immun. Meth., 62, 49-58, 1983.
Poirier et al, Nature (London), 270, 186, 1977.

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