Monoclonal antibody assay for Listeria monocytogenes

Details Monoclonal antibody assay for Listeria monocytogenes Monoclonal antibody assay for Listeria monocytogenes

1991-09-23

1994-03-15

Kepplinger, Esther L.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving antigen-antibody binding, specific binding protein...

435 792, 435 794, 435 795, 43524027, 5303884, 5303911, 5303913, G01N 33569, C12N 518, C07K 1528

Patent

active

052945375

ABSTRACT:
Mouse monoclonal antibodies which will specifically recognize the pathogen Listeria monocytogenes were produced by fusion of spleen cells from an animal immunized with live L. monocytogenes to an NS-1 myeloma partner, and three hybridomas were identified upon subsequent subcloning, Mab 20-10-2, Mab 36-6-12 and Mab 56-9-16 which were preferentially reactive with L. monocytogenes in a direct binding ELISA assay. An indirect "sandwich" assay was developed and used to further confirm the reactivity of these hybridomas using four serotypes of L. monocytogenes and other common cross reacting bacteria.

REFERENCES:
patent: 4950589 (1990-08-01), Butman et al.
Harlow et al, Antibodies A Laboratory Manual p. 372, Cold Spring Harbor Laboratory (1988).
Leinonen et al, Infect. Immun., 38(3):1203-1207 (Dec. 1982).
Voller et al, "Enzyme-Linked Immunosorbent Assay" in Manual of Clinical Laboratory Immunology pp. 99-109 (1986).
McLauchlin et al. J. Clin. Pathol. 41:983-988 (Sep. 1988).
Spitz et al, J. Immunol. Methods, 70:39-43 (1984).
Butman et al, Appl. Environ. Microbiol., 54(6) Jun. 1988) pp. 1564-1569.
Farber et al. J. Food Protection 50(6):479-484 (Jun. 1987).

Affiliated with

Also associated with

Rating

Monoclonal antibody assay for Listeria monocytogenes does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Monoclonal antibody assay for Listeria monocytogenes, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Monoclonal antibody assay for Listeria monocytogenes will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-1535424