Drug – bio-affecting and body treating compositions – Immunoglobulin – antiserum – antibody – or antibody fragment,... – Binds antigen or epitope whose amino acid sequence is...
Patent
1998-01-15
2000-10-17
Kemmerer, Elizabeth
Drug, bio-affecting and body treating compositions
Immunoglobulin, antiserum, antibody, or antibody fragment,...
Binds antigen or epitope whose amino acid sequence is...
4241411, 435 71, A61K 39395, G01N 3353
Patent
active
061327190
DESCRIPTION:
BRIEF SUMMARY
TECHNICAL FIELD
The present invention relates to a novel monoclonal antibody, and an immunological assay of a D-dimer produced by digesting human stabilized fibrin with granulocyte elastase (hereinafter sometimes referred to as "e-D-dimer") and a DD/E complex produced by digesting human stabilized fibrin with granulocyte elastase (hereinafter sometimes referred to as e-DD/E complex).
The monoclonal antibody according to the present invention is useful as a reagent for the immunological assay of the e-D-dimer and the e-DD/E complex which are produced in plasma or serum when human stabilized fibrin is digested with granulocyte elastase. The e-D-dimer and the e-DD/E complex are useful as a maker to predict postoperative disorder of multiple organs and pulmonary emphysema.
BACKGROUND ART
Digestion products of human stabilized fibrin with various proteases are useful as diagnostic markers in clinical diagnosis. For example, a D-dimer produced by digesting human stabilized fibrin with plasmin (hereinafter sometimes referred to as "p-D-dimer") and a DD/E complex produced by digesting human stabilized fibrin with plasmin (hereinafter sometimes referred to as "p-DD/E complex") are widely used as a diagnostic marker of disseminated intravascular coagulation (DIC). In determination of the p-D-dimer and p-DD/E complex in a sample from a living body, agglutination of latex sensitized with a monoclonal antibody specific to the p-D-dimer is generally used.
Further, the e-D-dimer and e-DD/E complex of human stabilized fibrin are useful as a marker to predict postoperative disorder of multiple organs and pulmonary emphysema. Agglutination reaction of latex sensitized with the above monoclonal antibody specific to the p-D-dimer makes it possible to determine the p-D-dimer and p-DD/E complex produced by the action of plasmin, but does not make it possible to determine the e-D-dimer and e-DD/E complex produced by the action of granulocyte elastase. Therefore, there have been some attempts to determine the e-D-dimer and e-DD/E complex of human stabilized fibrin.
A monoclonal antibody which reacts with an N-terminal site (A.alpha.22-36) newly formed on the Aa chain of fibrinogen when it is digested with granulocyte elastase was reported (Blood Coagulation and Fibrinolysis, vol. 6, p 259, 1995). The monoclonal antibody has an antigen-binding site in an E domain, and therefore, different from the monoclonal antibody according to the present invention. Further, the former monoclonal antibody slightly reacts with fibrinogen, and thus, digestion products of fibrinogen or fibrin with granulocyte elastase in plasma cannot be determined by EIA wherein the former monoclonal antibody is used.
Further, polyclonal antibodies prepared by adsorbing and removing antibodies which can react with plasmin-digested D-monomer, plasmin-digested D-dimer, plasmin-digested DD/E complex and fibrinogen from polyclonal antibodies obtained by immunization with granulocyte elastase-D-fragment of fibrinogen (J. Lab. Clin. Med. vol. 102, p 858, 1983) are sometimes used, but the procedure is cumbersome.
DISCLOSURE OF INVENTION
The object of the present invention is to provide an assay method of an amount of the e-D-dimer and e-DD/E complex in a sample from a living body, such as plasma, without an influence of amounts of fibrinogen, plasmin-digested products of fibrinogen, or plasmin-digested products of human stabilized fibrin (particularly, p-D-dimer or p-DD/E complex) which are expected to co-exist therewith in the sample.
Other objects and advantages will be apparent from the following description.
According to the present invention, there is provided a monoclonal antibody which specifically reacts with a D-monomer produced by digesting human fibrinogen with granulocyte elastase and D-domain containing digestion products produced by digesting human stabilized fibrin with granulocyte elastase, but does not react with fibrinogen, or a fragment X, Y or E produced by digesting fibrinogen with granulocyte elastase.
According to the present invention, the
Fukushi Ken
Inuzuka Kimiko
Ito Yumiko
Kohno Isao
Basi Nirmal S.
Iatron Laboratories Inc.
Kemmerer Elizabeth
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