Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Hydrolase
Patent
1987-12-02
1991-08-06
Kepplinger, Esther L.
Chemistry: molecular biology and microbiology
Enzyme , proenzyme; compositions thereof; process for...
Hydrolase
530387, 935104, 935108, 935110, 435 7021, 4351722, C12N 520, C07K 1528, C12P 2108
Patent
active
050377558
ABSTRACT:
A monoclonal antibody specific to Agrobacterium tumefaciens biovar 3. A method of diagnosing Agrobacterium tumefaciens biovar 3 associated grapevine disease comprising: (1) culturing bacteria from grapevine tissue suspected of being infected with Agrobacterium tumefaciens biovar 3; (2) reacting the bacteria with a monoclonal antibody specific to Agrobacterium tumefaciens biovar 3 under conditions sufficient to form an antigen-antibody complex between antigens specific to Agrobacterium tumefaciens biovar 3 and the monoclonal antibody; and (3) detecting the presence of the antigen-antibody complex. A method for diagnosing Agrobacterium tumefaciens biovar 3 associated grapevine disease from crown gall tissue comprising: (1) preparing separate suspensions of ground gall tissue to be diagnosed and of ground wood of the same cultivar as a control; (2) separately reacting specific to Agrobacterium tumefaciens biovar 3 under conditions sufficient to form an antigen-antibody complex between antigens specific to Agrobacterium tumefaciens biovar 3 and the monoclonal antibody; (3) assaying for the presence of the antigen-antibody complex ; and (4) comparing the assay results for the gall tissue to be diagnosed to the assays results for the wood control. A method for diagnosing agrobacterium tumefaciens biovar 3 associated grapevine disease from nonsymptomatic grapevine cuttings comprising: (1) separately flushing fluid through cuttings to be diagnosed and through uninfected control cuttings; (2) separately reactign the fluid flushed through the cuttings with monoclonal antiody specific to agrobacterium tumefaciens biovar 3 under conditions sufficient to form an antigen-antibody complex between antigens specific to Agrobacterium tumefaiens biovar 3 and the monoclonal antibody; (3) assaying for the presence of the antigen-antibody complex; and (4) comparing the assay results for said cuttings to be diagnosed to the assay results for the control cuttings. A hybridoma that secretes the above-described monoclonal antibody and a method of producing the hybridoma.
REFERENCES:
Bazzi et al. Phytopath Medit. 27:51-56 1988.
Bazzi, C. et al. "Detection of Agrobacterium Tumefaciens in Grapevine Cuttings", Bull OEPP (Organ. Eur. Mediterr. Prot. Plant.) 17(1): 105-112, 1987, Cited in Dialog, Accession #0017541887, Brosis Number: 84018947.
Burr, T. J. et al., "Isolation of Agrobacterium Tumefaciens Biovar3 from Grapevine Galls and Sapand from Vineyard Soil", Phytopathology 73(20:163-165, 1983, Cited in Dialog, Accession #0013333181, Brosis Number: 76090673.
International Journal of Systematic Bacteriology, Jul. 1990, pp. 236-241, vol. 40, No. 3, Ophel and Kerr, for "Agrobacterium Vitis Sp. Nov. for Strains of Agrobacterium Biovar 3 from Grapevines".
Bishop Andrew L.
Burr Thomas J.
Mittak Veronica L.
Cornell Research Foundation Inc.
Hutzell Paula
Kepplinger Esther L.
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