Monoclonal antibody

Details Monoclonal antibody Monoclonal antibody

1995-03-15

1997-08-19

Reisee, Lila

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving antigen-antibody binding, specific binding protein...

435 721, 435 723, 435330, 435331, 436 63, 436 64, 5303882, 5303888, 5303877, 5303879, G01N 3353, G01N 33542, G01N 33567, C07K 1600

Patent

active

056587422

ABSTRACT:
This invention relates to a monoclonal antibody having affinity to human TCF-II without any affinity to HGF and determination or purification methods of TCF-II using the antibody. The monoclonal antibody can be obtained by adding a blocking solution containing a surface active agent to the culture medium containing monoclonal antibody to block antibodies other than said monoclonal antibody, followed by reaction with a solid phase antigen, TCF-II. Human TCF-II can be selectively and effectively purified or determined without any influence of the presence of HGF by using the antibody.

REFERENCES:
E. Gohda et al., "Purification and Partial Characterization of Hepatocyte Growth Factor from Plasma of a Patient with Fulminant Hepatic Failure" J. Clin. Invest. 81:414-419 (1988).
K. Matsumoto et al., "Roles of HGF as a Pleiotropic Factor in Organ Regeneration", Hepatocyte Growth Factor-Scatter Factor (HGF-SF) and the C-Met Receptor, ed. I.D. Goldberg and E.M. Rosen, Birkhauser Verlag, Basel, Switzerland (1993).
K. Miyazawa et al., "Molecular Cloning and Sequence Analysis of cDNA for Human Hepatocyte Growth Factor" Biochem. and Biophys. Res. Comm. 163(2):967-973 (1989).
T. Seki et al., "Isolation and Expression of cDNA for Different Forms of Hepatocyte Growth Factor from Human Leukocyte" Biochem. and Biophys. Res. Comm. 172(1):321-327 (1990).
N. Shima et al., "Hepatocyte Growth Factor and Its Variant with a Deletion of Five Amino Acids Are Distinguishable in Their Biological Activity and Tertiary Structure" Biochem. and Biophys. Res. Comm. 200(2):808-815 (1994).
G. Shiota et al., "Hepatocyte Growth Factor Inhibits Growth of Hepatocellular Carcinoma Cells" Proc. Natl. Acad. Sci. USA 89:373-377 (1992).
H. Tajima et al., "Hepatocyte Growth Factor Has Potent Anti-Proliferative Activity in Various Tumor Cell Lines" FEBS 291(2):229-232 (1991).
K.M. Weidner et al., "Evidence for the Identity of Human Scatter Factor and Human Hepatocyte Growth Factor" Proc. Natl. Acad. Sci. USA 88:7001-7005 (1991).
N. Shima et al., "ELISA for F-TCF (human hepatocyte growth factor/hHGF)/fibroblast-derived tumor cytotoxic factor antigen employing monoclonal antibodies and its application to patients with liver diseases" Gastroenterologia Japonica 26(4):477-482 (1991).
Rubin et al, "A broad-spectrum human lung fibroblast-derived mitogen is a variant of hepatocyte growth factor" PNAS, vol. 88, pp. 415-419. Jan. 1991.
Smith et al, "Pitfalls in the use of ELISA to screen for monoclonal antibodies raised against small peptides" J. of Immunol. Methods, vol. 158, pp. 151-160 1993.
Shima et al., "Cytotoxic Factor/Hepatocyte Growth Factor from Human Fibroblasts: Cloning of Its cDNA, Purification and Characterization of Recombinant Protein" Biochem. and Biophys. Res. Comm. 180 No. 2:1151-1158 (1991).
K. Matsumoto et al., "Deletion of Kringle Domains or the N-Terminal Hairpin Structure in Hepatocyte Growth Factor Results in Marked Decreases in Related Biological Activities" Biochem. and Biophys. Res. Comm. 181 No. 2:691-699 (1991).

Affiliated with

Also associated with

Rating

Monoclonal antibody does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Monoclonal antibody, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Monoclonal antibody will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-1103880