Monoclonal antibodies which specifically recognize galactosyl-gl

Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Blood proteins or globulins – e.g. – proteoglycans – platelet...

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43524027, 435 723, 53038815, 5303913, 5303888, 424 9, 424 858, A61K 3766, C12H 500, C61K 3514

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active

051732920

ABSTRACT:
This invention provides a composition useful for diagnosing cancer which specifically recognizes a galactosyl-globoside. Preferably, the composition is a monoclonal antibody which specifically recognize the galactosyl-globoside antigen, gal-Gb4.
Additionally, the present invention provides two hybridoma cell lines, designated J309 and D579, which produce monoclonal antibodies that specifically recognize gal-Gb4.
The invention also provides a method of diagnosing a carcinoma which comprises contacting a sample from a human subject with a composition which specifically recognizes a galactosyl-globoside antigen and is labeled with a detectable moiety, under suitable conditions so as to form a detectable complex. The amount of formed complex is then quantified and correlated with values obtained from subjects devoid of carcinomas.
Another aspect of the invention provides a method of treating carcinoma which comprises administering an effective amount of galactosyl-globoside or an analog thereof and a pharmaceutically carrier to stimulate the body production of antibodies.

REFERENCES:
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patent: 4885358 (1989-12-01), Kannagi et al.
patent: 4906562 (1990-03-01), Hellstrom et al.
Young, et al.; Journal of Biological Chemistry, vol. 256 (21), pp. 10967-10972 (1981).
Bremer et al., Journal of Biological Chemistry, vol. 259 (23) pp. 14773-14777 (1984).
Kannagi et al.; Journal of Biological Chemistry, vol. 258 (14) pp. 8934-8942 (1983).
Shevinsky et al.; Cell, vol. 30, pp. 697-706 (1982).

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