Monoclonal antibodies which discriminate between strains of citr

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving virus or bacteriophage

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435 71, 435 7021, 43524027, 5303883, C12Q 170

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active

051047896

ABSTRACT:
Monoclonal cell lines producing antibodies to severe citrus tristeza virus have been developed. The antibodies react with crude plant extracts infected with severe isolates, but not with crude plant extracts infected with mild isolaates or healthy plant tissue. This immunological probe has been utilized in enzyme-linked immunosorbent assays as a rapid technique for identifying citrus infected with severe isolates of CTV. Field tests on recently planted trees in a commercial citrus grove have verified the effectiveness of the immunoassay.

REFERENCES:
Gumpf et al.: Production & Evaluation of Specific Monoclonal Antibodies to Citrus Tristenza Virus Strains, Phytophylactica, vol. 19 (2), 1987 pp. 159-162.
C. Vela et al., "Production and Characterization of Monoclonal Antibodies Specific for Citrus Tristeza Virus and Their Use for Diagnosis," J. Gen. Virol. 67: 91-96 (1986).
S. M. Garnsey et al., "Serological Titer of Citrus Tristeza Virus (CTV) Isolates of Varying Severity in Different Citrus Hosts," Phytopathology 75: 1311 (Abstract) (1985).
D. J. Gumpf et al., "Production and Evaluation of Specific Monoclonal Antibodies to Citrus Tristeza Virus Strains," Phytophylactica 19(2): i, 159-161 (Jun. 1987) (complete copy).

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