Monoclonal antibodies to vitamin B.sub.6 and immunoassay method

Details Monoclonal antibodies to vitamin B.sub.6 and immunoassay method Monoclonal antibodies to vitamin B.sub.6 and immunoassay method

1982-09-27

1984-08-14

Padgett, Benjamin R.

Chemistry: analytical and immunological testing

Biospecific ligand binding assay

Utilizing isolate of tissue or organ as binding agent

436505, 436527, 436531, 436548, 436804, 436808, 436810, 436815, 260112R, 260112B, 435 4, 435 7, 435 68, 4351721, 435240, 435810, 435948, 4351722, 424 85, 424 88, 424177, 935 89, 935 93, C07G 700

Patent

active

044657765

ABSTRACT:
A continuous hybridoma cell line which secretes recoverable quantities of monoclonal antibodies having specificity against Vitamin B.sub.6, which antibodies are useful in a method for detecting the presence of vitamin B.sub.6 in an animal sample.

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Kitter, Jason M., Dace Viceps-Madore, John A. Cidlowski, and J. W. Thanassi "Immunobolt Detection of Pyridoxal Phosphate Binding Proteins in Liver and Hepatoma Cytosolic Extracts", Biochem. Biophys. Res. Comm. 112(1):61(1983).
Kitter, Jason M., N. T. Meisler, D. Viceps-Madore, J. A. Cidlowski and J. W. Thanassi, "A General Immunochemical Method for Detecting Proteins on Blots", Analytical Biochemistry in press (1984).
Viceps-Madore, Dace, J. A. Cidlowski, J. M. Kittler and J. W. Thanassi, "Preparation, Characterization and Use of Monoclonal Antibodies to Vitamin B.sub.6 " J. Biological Chemistry 258:2689 (1983).

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