Monoclonal antibodies to skin cells

Details Monoclonal antibodies to skin cells Monoclonal antibodies to skin cells

1983-03-31

1987-03-10

Warren, Charles F.

Chemistry: molecular biology and microbiology

Spore forming or isolating process

436548, 435 68, 4351722, 435948, 935104, G01N 3354, C12N 500, C12N 1500, C12R 191

Patent

active

046491155

ABSTRACT:
Successive layers of guinea pig epidermis display discrete antigenic markers that are recognized by a selected panel of five monoclonal antibodies produced by hybridomas resulting from immunization of mice with suspensions of dissociated viable guinea pig epidermal cells. Antigen to Gpsk-1 marks the basement membrane, which membrane is probably produced by basal cells. Antigen to Gpsk-2 is expressed by basal and suprabasal cells. Antigens to both Gpsk-3 and Gpsk-4 occur on the spinous and overlying layers but are distinguished by differences in their representation on certain non-epidermal cell types and on other epithelia. Antigen to Gpsk-5 is found on basement membrane and on spinous and overlying granular and horny cells. Antigens to Gpsk-2 through 5 are situated at the cell surface and may recognize integral plasma membrane molecules expressed in differentiative sequence. The five antibodies differ also in their antigenic marker distribution among epithelial as well as other selected tissue types. These findings provide a background to potential dermatological applications of monoclonal antibodies to human epidermis.

REFERENCES:
Gigi et al., "Detection of a Cytokeratin Determinant Common to Diverse Epithelial Cells by a Broadly Cross-Reacting Monoclonal", EMBO Journal 1(11) pp. 1429-1437 (1982).
Eichner et al., "Expression of Keratin Antigens During Normal Epidermal Differentiation: Monoclonal Antibodies Studies", Journal of Investigative Dermatology 78(4) p. 341 (1982).
Grimm et al., "Hybridoma Antibodies to A431 Cell Plasma Membranes: Tests for Specific Immunoprecipitation of EGF Receptors", Monoclonal Antibodies in Endocrine Research ed. Fellows et al., Raven Press NY, pp. 99-110 (1981).
Woodcock-Mitchell et al., "Immunolocalization of Keratin Polypeptides in Human Epidermis Using Monoclonal Antibodies", Journal of Cell Biology 95 pp. 580-588 (1982).

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